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Literature summary for 1.18.6.1 extracted from

  • Fisher, K.; Newton, W.E.
    Nitrogenase proteins from Gluconacetobacter diazotrophicus, a sugarcane-colonizing bacterium (2005), Biochim. Biophys. Acta, 1750, 154-165.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of nitrogenase in the absence of NH4+ and at initial O2 concentrations above 5% in the culture atmosphere Gluconacetobacter diazotrophicus

Inhibitors

Inhibitors Comment Organism Structure
NaCl half-maximal inhibition at 100 mM Gluconacetobacter diazotrophicus

Metals/Ions

Metals/Ions Comment Organism Structure
Iron component Gd1, iron and molybdenum in a 12:1 ratio. Component Gd2, 3.9 Fe atoms per molecule Gluconacetobacter diazotrophicus
Molybdenum component Gd1, iron and molybdenum in a 12:1 ratio, molybdenum content is 1.4 atoms per enzyme tetramer Gluconacetobacter diazotrophicus

Organism

Organism UniProt Comment Textmining
Gluconacetobacter diazotrophicus
-
expression of nitrogenase in the absence of NH4+ and at initial O2 concentrations above 5% in the culture atmosphere
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6
-
30°C, pH 7.5 Gluconacetobacter diazotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
C2H2 + ?
-
Gluconacetobacter diazotrophicus H2 + ?
-
?

Subunits

Subunits Comment Organism
? component Gd1, subunits of 53000 and 57500 Da, component Gd2, subunits of 33000 Da, SDS-PAGE Gluconacetobacter diazotrophicus
More enzyme consists of the two components Gd1 and Gd2, each containing two subunits. Half-amximal catalytic activity for Gd1 is reached at a Gd1/Gd2 ratio of 4:1 Gluconacetobacter diazotrophicus

pH Range

pH Minimum pH Maximum Comment Organism
7.3
-
narrow range Gluconacetobacter diazotrophicus