Cloned (Comment) | Organism |
---|---|
expression of nitrogenase in the absence of NH4+ and at initial O2 concentrations above 5% in the culture atmosphere | Gluconacetobacter diazotrophicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | half-maximal inhibition at 100 mM | Gluconacetobacter diazotrophicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | component Gd1, iron and molybdenum in a 12:1 ratio. Component Gd2, 3.9 Fe atoms per molecule | Gluconacetobacter diazotrophicus | |
Molybdenum | component Gd1, iron and molybdenum in a 12:1 ratio, molybdenum content is 1.4 atoms per enzyme tetramer | Gluconacetobacter diazotrophicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconacetobacter diazotrophicus | - |
expression of nitrogenase in the absence of NH4+ and at initial O2 concentrations above 5% in the culture atmosphere | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.6 | - |
30°C, pH 7.5 | Gluconacetobacter diazotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
C2H2 + ? | - |
Gluconacetobacter diazotrophicus | H2 + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | component Gd1, subunits of 53000 and 57500 Da, component Gd2, subunits of 33000 Da, SDS-PAGE | Gluconacetobacter diazotrophicus |
More | enzyme consists of the two components Gd1 and Gd2, each containing two subunits. Half-amximal catalytic activity for Gd1 is reached at a Gd1/Gd2 ratio of 4:1 | Gluconacetobacter diazotrophicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
narrow range | Gluconacetobacter diazotrophicus |