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Literature summary for 1.18.6.1 extracted from

  • Rubio, L.M.; Singer, S.W.; Ludden, P.W.
    Purification and characterization of NafY (apodinitrogenase gamma subunit) from Azotobacter vinelandii (2004), J. Biol. Chem., 279, 19739-19746.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of Azotobacter vinelandii NafY protein in Escherichia coli strain BL21(DE3) Azotobacter vinelandii

Metals/Ions

Metals/Ions Comment Organism Structure
Iron enzyme contains an iron-molybdenum cofactor Azotobacter vinelandii
Molybdenum enzyme contains an iron-molybdenum cofactor Azotobacter vinelandii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
FeMo cofactor-NafY protein complex, gel filtration Azotobacter vinelandii
46000
-
NafY protein, gel filtration, sedimentation equilibrium centrifugation Azotobacter vinelandii

Organism

Organism UniProt Comment Textmining
Azotobacter vinelandii
-
-
-
Azotobacter vinelandii DJ
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Azotobacter vinelandii NafY protein from Escherichia coli strain BL21(DE3) Azotobacter vinelandii

Subunits

Subunits Comment Organism
dimer 2 * 26141-28000, unassociated NafY protein, sequence calculation and sedimentation equilibrium centrifugation Azotobacter vinelandii
More the NafY protein monomerizes upon binding to the FeMo cofactor Azotobacter vinelandii

Synonyms

Synonyms Comment Organism
More the nitrogenase complex is composed of 2 oxygen-labile metalloproteins: dinitrogenase and dinitrogenase reductase Azotobacter vinelandii

Cofactor

Cofactor Comment Organism Structure
iron-molybdenum cofactor required for activity of the enzyme complex, insertion in the presynthesized apodinitrogenase involving the monomeric 26 kDa NafY protein, which binds the FeMo cofactor with very high affinity via its HIs121, the cofactor-NafY-complex exhibits an EPR signal similar to isolated FeMo cofactor and the M-center of the enzyme, NafY also binds the biosynthetic precursor of the MoFe cofactor, the NifB-cofacor, determination of binding sites Azotobacter vinelandii