Activating Compound | Comment | Organism | Structure |
---|---|---|---|
molybdate | induces MoFe protein expression | Rhodobacter capsulatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tungstate | 30% inhibition at 0.01 mM, 50% inhibition at 0.1 mM, 95% inhibition at 1 mM, inhibits MoFe protein expression | Rhodobacter capsulatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | EPR signals of Mo and of W containing cofactor, redox potentials | Rhodobacter capsulatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | required, part of the MoFe protein cofactor | Rhodobacter capsulatus | |
Molybdenum | required, part of the MoFe protein cofactor, can be partially, about 25%, substituted by tungsten, Mo content quantification, overview, Mo cannot be substituted by rhenium | Rhodobacter capsulatus | |
additional information | rhenium cannot substitute for molybdenum in vitro, nor in vivo | Rhodobacter capsulatus | |
Tungsten | can partially, about 25%, substitute for molybdenum, isolation of a tungsten-substituted enzyme after growth of the cells in tungsten-supplemented and molybdenum-deficient medium | Rhodobacter capsulatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
200000 | 250000 | tungsten-substituted enzyme component, gel filtration | Rhodobacter capsulatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodobacter capsulatus | - |
wild-type strain B10S, molybdenum-containing, nif-encoded enzyme variant | - |
Purification (Comment) | Organism |
---|---|
wild-type and tungsten-substituted enzymes | Rhodobacter capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylene + ? | - |
Rhodobacter capsulatus | ethylene + ? | - |
? | |
ethylene + ? | - |
Rhodobacter capsulatus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | tungsten-substituted enzyme component | Rhodobacter capsulatus |
Synonyms | Comment | Organism |
---|---|---|
dinitrogenase | - |
Rhodobacter capsulatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
iron-molybdenum cofactor | - |
Rhodobacter capsulatus |