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Literature summary for 1.18.6.1 extracted from

  • Siemann, S.; Schneider, K.; Oley, M.; Muller, A.
    Characterization of a tungsten-substituted nitrogenase isolated from Rhodobacter capsulatus (2003), Biochemistry, 42, 3846-3857.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
molybdate induces MoFe protein expression Rhodobacter capsulatus

Inhibitors

Inhibitors Comment Organism Structure
tungstate 30% inhibition at 0.01 mM, 50% inhibition at 0.1 mM, 95% inhibition at 1 mM, inhibits MoFe protein expression Rhodobacter capsulatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information EPR signals of Mo and of W containing cofactor, redox potentials Rhodobacter capsulatus

Metals/Ions

Metals/Ions Comment Organism Structure
Iron required, part of the MoFe protein cofactor Rhodobacter capsulatus
Molybdenum required, part of the MoFe protein cofactor, can be partially, about 25%, substituted by tungsten, Mo content quantification, overview, Mo cannot be substituted by rhenium Rhodobacter capsulatus
additional information rhenium cannot substitute for molybdenum in vitro, nor in vivo Rhodobacter capsulatus
Tungsten can partially, about 25%, substitute for molybdenum, isolation of a tungsten-substituted enzyme after growth of the cells in tungsten-supplemented and molybdenum-deficient medium Rhodobacter capsulatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
200000 250000 tungsten-substituted enzyme component, gel filtration Rhodobacter capsulatus

Organism

Organism UniProt Comment Textmining
Rhodobacter capsulatus
-
wild-type strain B10S, molybdenum-containing, nif-encoded enzyme variant
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and tungsten-substituted enzymes Rhodobacter capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylene + ?
-
Rhodobacter capsulatus ethylene + ?
-
?
ethylene + ?
-
Rhodobacter capsulatus ?
-
?

Subunits

Subunits Comment Organism
tetramer tungsten-substituted enzyme component Rhodobacter capsulatus

Synonyms

Synonyms Comment Organism
dinitrogenase
-
Rhodobacter capsulatus

Cofactor

Cofactor Comment Organism Structure
iron-molybdenum cofactor
-
Rhodobacter capsulatus