Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of 2 mutants strain: 1 kanamycin-resistant with a deletion in NifHDK and 1 kanamycin, gentamycin, and molybdenum-resistant with double deletion in nif HDK and modABCD | Rhodobacter capsulatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Rhodobacter capsulatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | the iron-only enzyme consists of 2 components: Fe protein and FeFe protein, the latter contains 26 Fe atoms per molecul of protein | Rhodobacter capsulatus | |
additional information | the iron-only nitrogenase form contains no molybdenum, vanadium or any other heterometal atom | Rhodobacter capsulatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
13500 | - |
FeFe protein of iron-only enzyme: 2 * 59000 + 2 * 51000 + 2 * 13500, alpha2beta2gamma2, SDS-PAGE | Rhodobacter capsulatus |
51000 | - |
FeFe protein of iron-only enzyme: 2 * 59000 + 2 * 51000 + 2 * 13500, alpha2beta2gamma2, SDS-PAGE | Rhodobacter capsulatus |
57000 | - |
MoFe protein: 2 * 59000 + 2 * 57000, alpha2beta2, SDS-PAGE | Rhodobacter capsulatus |
59000 | - |
MoFe protein: 2 * 59000 + 2 * 57000, alpha2beta2, SDS-PAGE | Rhodobacter capsulatus |
59000 | - |
FeFe protein of iron-only enzyme: 2 * 59000 + 2 * 51000 + 2 * 13500, alpha2beta2gamma2, SDS-PAGE | Rhodobacter capsulatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | Rhodobacter capsulatus | - |
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? | |
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | Rhodobacter capsulatus B10S | - |
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodobacter capsulatus | - |
- |
- |
Rhodobacter capsulatus | - |
nitrogen fixation complex is encoded on nif gene cluster | - |
Rhodobacter capsulatus | - |
iron-only nitrogenase and molybdenum nitrogenase | - |
Rhodobacter capsulatus B10S | - |
- |
- |
Purification (Comment) | Organism |
---|---|
iron-only nitrogenase, both components | Rhodobacter capsulatus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | iron-only enzyme is composed of 2 components: FeFe protein and Fe protein | Rhodobacter capsulatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rhodobacter capsulatus |
0.235 | - |
substrate N2, purified Mo nitrogenase | Rhodobacter capsulatus |
0.26 | - |
substrate C2H2, iron-only nitrogenase with Fe protein to FeFe protein ratio of 40:1 | Rhodobacter capsulatus |
0.35 | - |
substrate N2, iron-only nitrogenase with Fe protein to FeFe protein ratio of 40:1 | Rhodobacter capsulatus |
1.2 | - |
substrate C2H2, purified Mo nitrogenase | Rhodobacter capsulatus |
1.3 | - |
substrate H+, purified Mo nitrogenase | Rhodobacter capsulatus |
2.4 | - |
substrate H+, iron-only nitrogenase with Fe protein to FeFe protein ratio of 40:1 | Rhodobacter capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O | - |
Rhodobacter capsulatus | 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate | - |
? | |
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O | - |
Rhodobacter capsulatus B10S | 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate | - |
? | |
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | - |
Rhodobacter capsulatus | 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? | |
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | - |
Rhodobacter capsulatus B10S | 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? | |
reduced ferredoxin + H+ + ATP | H2-producing activity is much higher in the iron-only enzyme form than in the molybdenum containing form and is less inhibited by competitive substrates | Rhodobacter capsulatus | oxidized ferredoxin + H2 + ADP + phosphate | - |
? | |
reduced ferredoxin + H+ + ATP | H2-producing activity is much higher in the iron-only enzyme form than in the molybdenum containing form and is less inhibited by competitive substrates | Rhodobacter capsulatus B10S | oxidized ferredoxin + H2 + ADP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | MoFe protein: 2 * 59000 + 2 * 57000, alpha2beta2, SDS-PAGE | Rhodobacter capsulatus |
? | FeFe protein of iron-only enzyme: 2 * 59000 + 2 * 51000 + 2 * 13500, alpha2beta2gamma2, SDS-PAGE | Rhodobacter capsulatus |
? | Fe protein of both enzyme forms: 2 * 30000-31000, gamma2, SDS-PAGE | Rhodobacter capsulatus |
tetramer | - |
Rhodobacter capsulatus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | below and above no remaining activity | Rhodobacter capsulatus |