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Literature summary for 1.18.6.1 extracted from
- The nitrogen-fixing system of Corynebacterium autotrophicum. Purification and properties of the nitrogenase components and two ferredoxins (1978), Eur. J. Biochem., 86, 133-142.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | - |
Xanthobacter autotrophicus |  |
| Molybdenum | - |
Xanthobacter autotrophicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Xanthobacter autotrophicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | Xanthobacter autotrophicus | - |
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Xanthobacter autotrophicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| both components | Xanthobacter autotrophicus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein | Xanthobacter autotrophicus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.07 | - |
purified MoFe protein | Xanthobacter autotrophicus |
| 1.26 | - |
purified Fe protein | Xanthobacter autotrophicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O | - |
Xanthobacter autotrophicus | 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate | - |
? | |
| 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | - |
Xanthobacter autotrophicus | 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | - |
Xanthobacter autotrophicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.1 | 7.3 | with substrates: N2, C2H2 | Xanthobacter autotrophicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.3 | pH 6.5: no activity below, pH 8.3: about 70% of activity maximum | Xanthobacter autotrophicus |
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