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Literature summary for 1.18.6.1 extracted from
- Isolation and characterization of nitrogenase from Klebsiella pneumoniae (1986), Methods Enzymol., 118, 511-519.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | - |
Klebsiella pneumoniae |  |
| Mg2+ | Mg2+ required for MgATP complex | Klebsiella pneumoniae | |
| Molybdenum | - |
Klebsiella pneumoniae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | Klebsiella pneumoniae | - |
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella pneumoniae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| both components | Klebsiella pneumoniae |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein | Klebsiella pneumoniae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.082 | - |
purified component II | Klebsiella pneumoniae |
| 2.16 | - |
purified component I | Klebsiella pneumoniae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O | - |
Klebsiella pneumoniae | 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate | - |
? | |
| 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | - |
Klebsiella pneumoniae | 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? | |
| dithionite + H+ + N2 + ATP | in vitro substrate | Klebsiella pneumoniae | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Klebsiella pneumoniae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Klebsiella pneumoniae |
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