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Literature summary for 1.18.6.1 extracted from

  • Burns, R.C.; Hardy, R.W.F.
    Purification of nitrogenase and crystallization of its Mo-Fe protein (1972), Methods Enzymol., 24, 480-496.
    View publication on PubMed
Search for more literature for 1.18.6.1

Crystallization (Commentary)

Crystallization (Comment) Organism
MoFe protein Azotobacter vinelandii
purified enzyme is diluted at room temperature with 3 volumes of Tris-HCl 0.01 M, pH 7.2, immediate crystal formation Azotobacter vinelandii

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Azotobacter vinelandii
1,2-Dihydroxybenzene 3,5-disulfonate
-
 Azotobacter vinelandii
2,2'-dipyridyl
-
 Azotobacter vinelandii
2,3-Dimercaptopropanol
-
 Azotobacter vinelandii
ADP
-
 Azotobacter vinelandii
CO
-
 Azotobacter vinelandii
Cu2+
-
 Azotobacter vinelandii
H2
-
 Azotobacter vinelandii
additional information high ionic strength inhibits Azotobacter vinelandii
NO
-
 Azotobacter vinelandii
O2
-
 Azotobacter vinelandii
Zn2+
-
 Azotobacter vinelandii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Azotobacter vinelandii
0.1
-
 N2
-
 Azotobacter vinelandii
0.3
-
 ATP
-
 Azotobacter vinelandii
0.4 1.2 C2H2
-
 Azotobacter vinelandii

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ divalent cation requirement is satisfied by Co2+, is best supported by concentrations of divalent cation one-half the concentration of ATP Azotobacter vinelandii
Fe2+ divalent cation requirement is satisfied by Fe2+, is best supported by concentrations of divalent cation one-half the concentration of ATP Azotobacter vinelandii
Mg2+ divalent metal requirement is satisfied by Mg2+, reaction is best supported by concentration of divalent cation one-half the concentration of ATP Azotobacter vinelandii
Mn2+ divalent cation requirement is satisfied by Mn2+, is best supported by concentrations of divalent cation one-half the concentration of ATP Azotobacter vinelandii
Ni2+ divalent cation requirement is satisfied by Ni2+, is best supported by concentrations of divalent cation one-half the concentration of ATP Azotobacter vinelandii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Azotobacter vinelandii
-
 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Azotobacter vinelandii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Azotobacter vinelandii

Reaction

Reaction Comment Organism Reaction ID
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Azotobacter vinelandii
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Azotobacter vinelandii

Storage Stability

Storage Stability Organism
-15°C, anaerobic storage, overnight, complete loss of activity Azotobacter vinelandii
22°C, O2-free atmosphere, pH 7-8, stable Azotobacter vinelandii
5°C, O2-free atmosphere, overnight, 80% loss of activity Azotobacter vinelandii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
 Azotobacter vinelandii 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
 ?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
 Azotobacter vinelandii 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O 1-propyne, 1-butyne and allene are reduced to the corresponding alkenes Azotobacter vinelandii 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
reduced ferredoxin + H+ + CH3NC + ATP
-
 Azotobacter vinelandii oxidized ferredoxin + CH4 + C2H4 + C3H6 + C3H8 + CH3NH2 + ADP + phosphate
-
 ?
reduced ferredoxin + H+ + N3- + ATP
-
 Azotobacter vinelandii oxidized ferredoxin + NH3 + N2 + ADP + phosphate
-
 ?