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Literature summary for 1.18.6.1 extracted from

  • Eady, R.R.
    Isolation and characterization of various nitrogenases (1980), Methods Enzymol., 69, 753-778.
No PubMed abstract available

Metals/Ions

Metals/Ions Comment Organism Structure
Iron
-
Paenibacillus polymyxa
Iron
-
Rhodospirillum rubrum
Iron
-
Bradyrhizobium lupini
Iron
-
Azotobacter chroococcum
Iron 17-19 atoms of iron per molecule of MoFe protein, overview Bradyrhizobium japonicum
Iron 17-19 atoms of iron per molecule of MoFe protein, overview Klebsiella pneumoniae
Iron 17-19 atoms of iron per molecule of MoFe protein, overview Clostridium pasteurianum
Iron 17-19 atoms of iron per molecule of MoFe protein, overview Azotobacter vinelandii
Iron 17-19 atoms of iron per molecule of MoFe protein, overview Allochromatium vinosum
Molybdenum 1-2 gatom per mol of MoFe protein , overview Bradyrhizobium japonicum
Molybdenum 1-2 gatom per mol of MoFe protein , overview Klebsiella pneumoniae
Molybdenum 1-2 gatom per mol of MoFe protein , overview Clostridium pasteurianum
Molybdenum 1-2 gatom per mol of MoFe protein , overview Azotobacter vinelandii
Molybdenum 1-2 gatom per mol of MoFe protein , overview Paenibacillus polymyxa
Molybdenum 1-2 gatom per mol of MoFe protein , overview Rhodospirillum rubrum
Molybdenum 1-2 gatom per mol of MoFe protein , overview Allochromatium vinosum
Molybdenum 1-2 gatom per mol of MoFe protein , overview Bradyrhizobium lupini
Molybdenum 1-2 gatom per mol of MoFe protein , overview Azotobacter chroococcum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000 74000 Fe protein: various methods, overview Bradyrhizobium japonicum
40000 74000 Fe protein: various methods, overview Klebsiella pneumoniae
40000 74000 Fe protein: various methods, overview Clostridium pasteurianum
40000 74000 Fe protein: various methods, overview Azotobacter vinelandii
40000 74000 Fe protein: various methods, overview Bradyrhizobium lupini
160000 270000 MoFe protein: various methods, overview Bradyrhizobium japonicum
160000 270000 MoFe protein: various methods, overview Klebsiella pneumoniae
160000 270000 MoFe protein: various methods, overview Clostridium pasteurianum
160000 270000 MoFe protein: various methods, overview Azotobacter vinelandii
160000 270000 MoFe protein: various methods, overview Allochromatium vinosum
160000 270000 MoFe protein: various methods, overview Bradyrhizobium lupini
160000 270000 MoFe protein: various methods, overview Azotobacter chroococcum
168000
-
MoFe protein, ultracentrifugation Clostridium pasteurianum
180000
-
MoFe protein, gel filtration Bradyrhizobium japonicum
194000
-
MoFe protein, gel filtration Bradyrhizobium lupini
200000
-
-
Bradyrhizobium japonicum
200000
-
-
Klebsiella pneumoniae
200000
-
MoFe protein, gel filtration Clostridium pasteurianum
216000
-
MoFe protein, gel filtration Azotobacter vinelandii
226000
-
MoFe protein, gel filtration Klebsiella pneumoniae
270000
-
MoFe protein, ultracentrifugation Azotobacter vinelandii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Bradyrhizobium japonicum
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Klebsiella pneumoniae
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Clostridium pasteurianum
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Azotobacter vinelandii
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Paenibacillus polymyxa
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Rhodospirillum rubrum
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Allochromatium vinosum
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Bradyrhizobium lupini
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Azotobacter chroococcum
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?

Organism

Organism UniProt Comment Textmining
Allochromatium vinosum
-
-
-
Azotobacter chroococcum
-
-
-
Azotobacter vinelandii
-
-
-
Bradyrhizobium japonicum
-
-
-
Bradyrhizobium lupini
-
-
-
Clostridium pasteurianum
-
-
-
Klebsiella pneumoniae
-
-
-
Paenibacillus polymyxa
-
-
-
Rhodospirillum rubrum
-
-
-

Oxidation Stability

Oxidation Stability Organism
extreme sensitivity to O2 Bradyrhizobium japonicum
extreme sensitivity to O2 Klebsiella pneumoniae
extreme sensitivity to O2 Clostridium pasteurianum
extreme sensitivity to O2 Azotobacter vinelandii
extreme sensitivity to O2 Paenibacillus polymyxa
extreme sensitivity to O2 Rhodospirillum rubrum
extreme sensitivity to O2 Allochromatium vinosum
extreme sensitivity to O2 Bradyrhizobium lupini
extreme sensitivity to O2 Azotobacter chroococcum
t1/2 Fe protein: 45 sec, t1/2 MoFe protein: 10 min Bradyrhizobium japonicum
t1/2 Fe protein: 45 sec, t1/2 MoFe protein: 10 min Klebsiella pneumoniae
t1/2 Fe protein: 45 sec, t1/2 MoFe protein: 10 min Azotobacter vinelandii
t1/2 Fe protein: 45 sec, t1/2 MoFe protein: 10 min Rhodospirillum rubrum
t1/2 Fe protein: 45 sec, t1/2 MoFe protein: 10 min Allochromatium vinosum
t1/2 Fe protein: 45 sec, t1/2 MoFe protein: 10 min Bradyrhizobium lupini
t1/2 Fe protein: 45 sec, t1/2 MoFe protein: 10 min Azotobacter chroococcum

Purification (Commentary)

Purification (Comment) Organism
-
Klebsiella pneumoniae
-
Clostridium pasteurianum
-
Azotobacter vinelandii
-
Allochromatium vinosum
-
Azotobacter chroococcum

Reaction

Reaction Comment Organism Reaction ID
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Bradyrhizobium japonicum
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Klebsiella pneumoniae
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Clostridium pasteurianum
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Azotobacter vinelandii
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Paenibacillus polymyxa
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Rhodospirillum rubrum
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Allochromatium vinosum
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Bradyrhizobium lupini
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Azotobacter chroococcum

Source Tissue

Source Tissue Comment Organism Textmining
root nodule bacteroid Bradyrhizobium japonicum
-
root nodule bacteroid Klebsiella pneumoniae
-
root nodule bacteroid Clostridium pasteurianum
-
root nodule bacteroid Azotobacter vinelandii
-
root nodule bacteroid Allochromatium vinosum
-
root nodule bacteroid Bradyrhizobium lupini
-
root nodule bacteroid Azotobacter chroococcum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Bradyrhizobium japonicum
additional information
-
-
Paenibacillus polymyxa
additional information
-
-
Rhodospirillum rubrum
additional information
-
-
Bradyrhizobium lupini
additional information
-
activity of Fe proteins and MoFe proteins Klebsiella pneumoniae
additional information
-
activity of Fe proteins and MoFe proteins Clostridium pasteurianum
additional information
-
activity of Fe proteins and MoFe proteins Azotobacter vinelandii
additional information
-
activity of Fe proteins and MoFe proteins Allochromatium vinosum
additional information
-
activity of Fe proteins and MoFe proteins Azotobacter chroococcum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
Bradyrhizobium japonicum 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
Klebsiella pneumoniae 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
Clostridium pasteurianum 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
Azotobacter vinelandii 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
Paenibacillus polymyxa 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
Rhodospirillum rubrum 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
Allochromatium vinosum 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
Bradyrhizobium lupini 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
Azotobacter chroococcum 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?

Subunits

Subunits Comment Organism
dimer Fe protein is a dimer of 2 identical subunits, MW 27500-34600, various methods, overview Bradyrhizobium japonicum
dimer Fe protein is a dimer of 2 identical subunits, MW 27500-34600, various methods, overview Klebsiella pneumoniae
dimer Fe protein is a dimer of 2 identical subunits, MW 27500-34600, various methods, overview Clostridium pasteurianum
dimer Fe protein is a dimer of 2 identical subunits, MW 27500-34600, various methods, overview Azotobacter vinelandii
dimer Fe protein is a dimer of 2 identical subunits, MW 27500-34600, various methods, overview Paenibacillus polymyxa
dimer Fe protein is a dimer of 2 identical subunits, MW 27500-34600, various methods, overview Rhodospirillum rubrum
dimer Fe protein is a dimer of 2 identical subunits, MW 27500-34600, various methods, overview Allochromatium vinosum
dimer Fe protein is a dimer of 2 identical subunits, MW 27500-34600, various methods, overview Bradyrhizobium lupini
dimer Fe protein is a dimer of 2 identical subunits, MW 27500-34600, various methods, overview Azotobacter chroococcum