Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.18.1.7 extracted from

  • Haigler, B.; Gibson, D.
    Purification and properties of ferredoxinNAP, a component of naphthalene dioxygenase from Pseudomonas sp. strain NCIB 9816 (1990), J. Bacteriol., 172, 465-468.
    View publication on PubMedView publication on EuropePMC

Metals/Ions

Metals/Ions Comment Organism Structure
Iron the protein contains 2 g atoms each of iron Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13600
-
x * 13600, SDS-PAGE Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida Q52126
-
-
Pseudomonas putida NCIB 9816 Q52126
-
-

Purification (Commentary)

Purification (Comment) Organism
Blue Sepharose column chromatography, DEAE-cellulose column chromatography, and Sephadex G-75 gel filtration Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.52
-
purified enzyme, in the absence of FAD and with NADPH as cosubstrate, pH and temperature not specified in the publication Pseudomonas putida
13.2
-
purified enzyme, in the absence of FAD and with NADH as cosubstrate, pH and temperature not specified in the publication Pseudomonas putida
36.7
-
purified enzyme, in the presence of FAD and NADPH, pH and temperature not specified in the publication Pseudomonas putida
71
-
purified enzyme, in the presence of FAD and NADH, pH and temperature not specified in the publication Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricytochrome c + NADH the enzyme has about 2fold higher activity with NADH compared to NADPH Pseudomonas putida 2 ferrocytochrome c + NAD+ + H+
-
?
2 ferricytochrome c + NADH the enzyme has about 2fold higher activity with NADH compared to NADPH Pseudomonas putida NCIB 9816 2 ferrocytochrome c + NAD+ + H+
-
?
oxidized cytochrome c + NADPH
-
Pseudomonas putida reduced cytochrome c + NADP+ + H+
-
?
oxidized cytochrome c + NADPH
-
Pseudomonas putida NCIB 9816 reduced cytochrome c + NADP+ + H+
-
?

Subunits

Subunits Comment Organism
? x * 13600, SDS-PAGE Pseudomonas putida

Synonyms

Synonyms Comment Organism
NADH-ferredoxinNAP reductase
-
Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
FAD preferred flavin cofactor Pseudomonas putida
NADH NADH is preferred over NADPH Pseudomonas putida
NADPH
-
Pseudomonas putida