Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetate | 50% inhibition at 10 mM | Pseudomonas putida | |
N-ethylmaleimide | 67% inhibition at 10 mM | Pseudomonas putida | |
o-phenanthroline | 63% inhibition at 10 mM | Pseudomonas putida | |
p-chloromercuribenzoate | 94% inhibition at 0.0005 mM | Pseudomonas putida | |
Sodium azide | 46% inhibition at 40 mM | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | the purified protein contains 1.8 g atoms of iron | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
1 * 36000, SDS-PAGE | Pseudomonas putida |
37000 | - |
gel filtration | Pseudomonas putida |
37104 | - |
1 * 37104, calculated from amino acid sequence | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | Q52126 | - |
- |
Pseudomonas putida NCIB 9816 | Q52126 | - |
- |
Purification (Comment) | Organism |
---|---|
Sepharose CL-6B column chromatography and DEAE-cellulose column chromatography | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
397 | - |
purified enzyme, pH and temperature not specified in the publication | Pseudomonas putida |
Storage Stability | Organism |
---|---|
-20°C, purified enzyme, 1 month, minimal loss of activity | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricyanide + NADH | best substrate | Pseudomonas putida | 2 ferrocyanide + NAD+ + H+ | - |
? | |
2 ferricyanide + NADH | best substrate | Pseudomonas putida NCIB 9816 | 2 ferrocyanide + NAD+ + H+ | - |
? | |
2 ferricytochrome c + NADH | - |
Pseudomonas putida | 2 ferrocytochrome c + NAD+ + H+ | - |
? | |
2 ferricytochrome c + NADH | - |
Pseudomonas putida NCIB 9816 | 2 ferrocytochrome c + NAD+ + H+ | - |
? | |
NADH + H+ + oxidized 2,6-dichlorophenolindophenol | - |
Pseudomonas putida | NAD+ + reduced 2,6-dichlorophenolindophenol | - |
? | |
NADH + H+ + oxidized 2,6-dichlorophenolindophenol | - |
Pseudomonas putida NCIB 9816 | NAD+ + reduced 2,6-dichlorophenolindophenol | - |
? | |
oxidized cytochrome c + NADPH | NADPH is also effective as an electron donor but yields only 39% of the activity obtained with NADH in the cytochrome c reductase assay | Pseudomonas putida | reduced cytochrome c + NADP+ + H+ | - |
? | |
oxidized cytochrome c + NADPH | NADPH is also effective as an electron donor but yields only 39% of the activity obtained with NADH in the cytochrome c reductase assay | Pseudomonas putida NCIB 9816 | reduced cytochrome c + NADP+ + H+ | - |
? | |
oxidized Nitro Blue tetrazolium + NADH | - |
Pseudomonas putida | reduced Nitro Blue tetrazolium + NAD+ + H+ | - |
? | |
oxidized Nitro Blue tetrazolium + NADH | - |
Pseudomonas putida NCIB 9816 | reduced Nitro Blue tetrazolium + NAD+ + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 36000, SDS-PAGE | Pseudomonas putida |
monomer | 1 * 37104, calculated from amino acid sequence | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
NADH-ferredoxinNAP reductase | - |
Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
20 | the purified enzyme is unstable at room temperature. It loses 50% of its cytochrome c reductase activity within the first 8 h. At 0 to 5°C, the purified enzyme retains 70% of its cytochrome c reductase activity after 5 days | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the enzyme binds 1 mol of FAD per mol of enzyme protein | Pseudomonas putida | |
NADH | - |
Pseudomonas putida |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas putida | calculated from amino acid sequence | - |
6.3 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
10 | - |
pH and temperature not specified in the publication | Pseudomonas putida | iodoacetate |