Cloned (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
additional information | expression of wild-type as His-tagged protein. Molecular, spectral, and electron transferring properties of recombinant His6-Pdr to artificial and native electron acceptors are similar to those of the wild-type protein. Contrary to wild-type, under anaerobic conditions, NAD+ induces in His6 Pdr spectral changes indicative of flavin reduction and formation of the charge transfer complex between the reduced FAD and NAD+ | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | P16640 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fe(CN)62- + NAD+ | - |
Pseudomonas putida | Fe(CN)63- + NADH + H+ | - |
? | |
additional information | wild-type and His6 Pdr are able to function as NAD(H)-dependent dithiol/disulfide oxidoreductases catalyzing both forward and reverse reactions, NAD+-dependent oxidation of thiols, and NADH-dependent reduction of disulfides. This function of the flavoprotein can be dissociated from electron transfer to putidaredoxin | Pseudomonas putida | ? | - |
? | |
reduced 2,6-dichlorophenolindophenol + NAD+ | - |
Pseudomonas putida | oxidized 2,6-dichlorophenolindophenol + NADH + H+ | - |
? | |
reduced putidaredoxin + NAD+ | - |
Pseudomonas putida | oxidized putidaredoxin + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | both WT and His6-Pdr undergo a monomer-dimer association-dissociation | Pseudomonas putida |
monomer | both WT and His6-Pdr undergo a monomer-dimer association-dissociation | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
Pdr | - |
Pseudomonas putida |
putidaredoxin reductase | - |
Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
126 | - |
Fe(CN)62- | pH 8.0, 25°C | Pseudomonas putida | |
264 | - |
reduced putidaredoxin | pH 8.0, 25°C | Pseudomonas putida | |
671 | - |
Fe(CN)62- | pH 8.0, 25°C | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | both WT and His6-Pdr are partially present as an NAD+-bound form | Pseudomonas putida |