Activating Compound | Comment | Organism | Structure |
---|---|---|---|
FAD | the enzyme contains one flavin adenine dinucleotide (FAD) molecule per mol | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
NADPH | pH 8.0, 80°C, cosubstrate benzyl viologen | Pyrococcus furiosus | |
0.01 | - |
oxidized rubredoxin | pH 8.0, 80°C, cosubstrate NADPH | Pyrococcus furiosus | |
0.034 | - |
NADH | pH 8.0, 80°C, cosubstrate benzyl viologen | Pyrococcus furiosus | |
0.05 | - |
oxidized rubredoxin | pH 8.0, 80°C, cosubstrate: NADPH | Pyrococcus furiosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no metals can be detected | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
1 * 45000, SDS-PAGE | Pyrococcus furiosus |
51000 | - |
gel filtration | Pyrococcus furiosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 oxidized rubredoxin + NADH | Pyrococcus furiosus | - |
2 reduced rubredoxin + NAD+ + H+ | - |
? | |
2 oxidized rubredoxin + NADPH | Pyrococcus furiosus | rubredoxin is the most efficient electron acceptor | 2 reduced rubredoxin + NADP+ + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20000 | - |
pH 8.0, 80°C | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 oxidized rubredoxin + NADH | - |
Pyrococcus furiosus | 2 reduced rubredoxin + NAD+ + H+ | - |
? | |
2 oxidized rubredoxin + NADPH | rubredoxin is the most efficient electron acceptor | Pyrococcus furiosus | 2 reduced rubredoxin + NADP+ + H+ | - |
? | |
additional information | the enzyme does not reduce Pyrococcus furiosus ferredoxin | Pyrococcus furiosus | ? | - |
? | |
oxidized benzyl viologen + NADH | - |
Pyrococcus furiosus | reduced benzyl viologen + NAD+ | - |
? | |
oxidized benzyl viologen + NADPH | - |
Pyrococcus furiosus | reduced benzyl viologen + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, SDS-PAGE | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
NROR | - |
Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
assay at | Pyrococcus furiosus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 80 | about 70fold increase in kcat/Km for oxidized rebredoxin increases from 25°C to 80°C | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
12 h, 50% loss of activity | Pyrococcus furiosus |
95 | - |
2 h, 50% loss of activity | Pyrococcus furiosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
221 | - |
oxidized rubredoxin | pH 8.0, 25°C, cosubstrate NADPH | Pyrococcus furiosus | |
341 | - |
NADPH | pH 8.0, 80°C, cosubstrate: benzyl viologen | Pyrococcus furiosus | |
354 | - |
NADH | pH 8.0, 80°C, cosubstrate: benzyl viologen | Pyrococcus furiosus | |
15000 | - |
oxidized rubredoxin | pH 8.0, 80°C, cosubstrate NADPH | Pyrococcus furiosus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10400 | - |
NADH | pH 8.0, 80°C, cosubstrate benzyl viologen | Pyrococcus furiosus | |
22050 | - |
oxidized rubredoxin | pH 8.0, 80°C, cosubstrate NADPH | Pyrococcus furiosus | |
68300 | - |
NADPH | pH 8.0, 80°C, cosubstrate benzyl viologen | Pyrococcus furiosus | |
300000 | - |
oxidized rubredoxin | pH 8.0, 80°C, cosubstrate NADPH | Pyrococcus furiosus |