Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.18.1.2 extracted from

  • Seo, D.; Asano, T.
    C-terminal residues of ferredoxin-NAD(P)+ reductase from Chlorobaculum tepidum are responsible for reaction dynamics in the hydride transfer and redox equilibria with NADP+/NADPH (2018), Photosynth. Res., 136, 275-290 .
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information deletions of Tyr326-Glu360 decrease the hydride transfer rate and the amount of reduced enzyme increases at equilibrium relative to wild type. Deletions of Phe337-Glu360 and Ser338-Glu360 result in slight changes in the reaction kinetics and redox equilibrium compared to the wild type enzyme Chlorobaculum tepidum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0142
-
NADPH with 2 mM ferricyanide in 20 mM HEPES-NaOH buffer, at pH 7.0 and 25°C Chlorobaculum tepidum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
91000
-
gel filtration Chlorobaculum tepidum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
reduced ferredoxin + NADP+ + H+ Chlorobaculum tepidum
-
oxidized ferredoxin + NADPH
-
r

Organism

Organism UniProt Comment Textmining
Chlorobaculum tepidum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ferricyanide + NADPH
-
Chlorobaculum tepidum ferrocyanide + NADP+ + H+
-
r
reduced ferredoxin + NADP+ + H+
-
Chlorobaculum tepidum oxidized ferredoxin + NADPH
-
r

Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE Chlorobaculum tepidum
? x * 39190, MALDI-TOF mass spectrometry Chlorobaculum tepidum
? x * 39240, calculated from amino acid sequence Chlorobaculum tepidum

Synonyms

Synonyms Comment Organism
ferredoxin-NAD(P)+ reductase
-
Chlorobaculum tepidum
FNR
-
Chlorobaculum tepidum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
213
-
NADPH with 2 mM ferricyanide in 20 mM HEPES-NaOH buffer, at pH 7.0 and 25°C Chlorobaculum tepidum

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Chlorobaculum tepidum
NADPH
-
Chlorobaculum tepidum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
15000
-
NADPH with 2 mM ferricyanide in 20 mM HEPES-NaOH buffer, at pH 7.0 and 25°C Chlorobaculum tepidum