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Literature summary for 1.18.1.2 extracted from

  • Nguyen, D.M.N.; Schut, G.J.; Zadvornyy, O.A.; Tokmina-Lukaszewska, M.; Poudel, S.; Lipscomb, G.L.; Adams, L.A.; Dinsmore, J.T.; Nixon, W.J.; Boyd, E.S.; Bothner, B.; Peters, J.W.; Adams, M.W.W.
    Two functionally distinct NADP+-dependent ferredoxin oxidoreductases maintain the primary redox balance of Pyrococcus furiosus (2017), J. Biol. Chem., 292, 14603-14616 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.18.1.2

Crystallization (Commentary)

Crystallization (Comment) Organism
vapor diffusion method, using 0.22 M magnesium sulfate, 27% (w/v) polyethylene glycol 3350, and 0.4% (v/v) ethyl acetate in the presence of 1 mM sodium dithionite Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
88951
-
 mass spectrometry Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
reduced ferredoxin + NADP+ + H+ Pyrococcus furiosus
-
 oxidized ferredoxin + NADPH
-
 ?
reduced ferredoxin + NADP+ + H+ Pyrococcus furiosus COM1
-
 oxidized ferredoxin + NADPH
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus I6TYZ3
-
-
Pyrococcus furiosus COM1 I6TYZ3
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Trap column chromatography, Ni-NTA column chromatography, and S100 gel filtration Pyrococcus furiosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17
-
 after 1fold purification, at pH 7.5 and 80°C Pyrococcus furiosus
275
-
 after 15fold purification, at pH 7.5 and 80°C Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme does not perform NADH-dependent benzyl viologen reduction Pyrococcus furiosus ?
-
 ?
additional information the enzyme does not perform NADH-dependent benzyl viologen reduction Pyrococcus furiosus COM1 ?
-
 ?
reduced benzyl viologen + NADP+ + H+
-
 Pyrococcus furiosus oxidized benzyl viologen + NADPH
-
 ?
reduced benzyl viologen + NADP+ + H+
-
 Pyrococcus furiosus COM1 oxidized benzyl viologen + NADPH
-
 ?
reduced ferredoxin + NADP+ + H+
-
 Pyrococcus furiosus oxidized ferredoxin + NADPH
-
 ?
reduced ferredoxin + NADP+ + H+
-
 Pyrococcus furiosus COM1 oxidized ferredoxin + NADPH
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 52498 + 1 * 34029, calculated from amino acid sequence Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
ferredoxin NADP oxidoreductase
-
 Pyrococcus furiosus
NfnIII
-
 Pyrococcus furiosus

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Pyrococcus furiosus
NADP+
-
 Pyrococcus furiosus
[2Fe-2S]-center
-
 Pyrococcus furiosus
[4Fe-4S]-center
-
 Pyrococcus furiosus

General Information

General Information Comment Organism
physiological function the enzyme is important for growth in the presence of sulfur Pyrococcus furiosus