Literature summary for 1.18.1.2 extracted from

Bowsher, C.G.; Eyres, L.M.; Gummadova, J.O.; Hothi, P.; McLean, K.J.; Munro, A.W.; Scrutton, N.S.; Hanke, G.T.; Sakakibara, Y.; Hase, T.
Identification of N-terminal regions of wheat leaf ferredoxin NADP+ oxidoreductase important for interactions with ferredoxin (2011), Biochemistry, 50, 1778-1787.

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Protein Variants

Protein Variants	Comment	Organism
additional information	study on impact of N-terminal truncation on interaction with ferredoxin using deletions of up to 25 amino acids. Similar kcat values for cytochrome c reduction are measured for all but the most truncated pFNRII[N-5]DEGV which contains a truncated N-terminal domain beginning after the KISKK domain. The longer forms of isoform FNRII bind more strongly to a ferredoxin affinity column than the shorter forms. Mutants bearing lysine-to-glutamine mutations in the KISKK domain are affected in binding to the ferredoxin column	Triticum aestivum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00205	-	ferricytochrome c	mutant bearing a lysine to glutamine mutation in the first lysine residue of the KISKK domain, pH 7.5, temperature not specified in the publication	Triticum aestivum
0.00312	-	ferricytochrome c	wild-type, pH 7.5, temperature not specified in the publication	Triticum aestivum
0.0035	-	ferricytochrome c	mutant bearing a lysine to glutamine mutation in the second lysine residue of the KISKK domain, pH 7.5, temperature not specified in the publication	Triticum aestivum
0.00999	-	ferricytochrome c	mutant bearing an N-terminal region with the full wheat KISKK domain, pH 7.5, temperature not specified in the publication	Triticum aestivum
0.0103	-	ferricytochrome c	mutant bearing a truncated N-terminal domain beginning after the KISKK domain, pH 7.5, temperature not specified in the publication	Triticum aestivum
0.0104	-	ferricytochrome c	mutant bearing lysine to glutamine mutations in the second and third lysine residues of the KISKK domain, pH 7.5, temperature not specified in the publication	Triticum aestivum
0.01103	-	ferricytochrome c	mutant with an extended N-terminal region to include the EAxxPA motif of maize, pH 7.5, temperature not specified in the publication	Triticum aestivum

Organism

Organism	UniProt	Comment	Textmining
Triticum aestivum	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Triticum aestivum	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 ferricytochrome c2 + NADPH	-	Triticum aestivum	2 ferrocytochrome c2 + NADP+ + H+	-	?
ferricytochrome c + NADP+	-	Triticum aestivum	ferrocytochrome c + NADPH + H+	-	r
ferrocytochrome c + NADP+	-	Triticum aestivum	ferricytochrome c + NADPH + H+	-	r

Synonyms

Synonyms	Comment	Organism
FNRII	-	Triticum aestivum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
38.1	-	reduced flavodoxin	mutant bearing a truncated N-terminal domain beginning after the KISKK domain, pH 7.5, temperature not specified in the publication	Triticum aestivum
45.4	-	reduced flavodoxin	mutant bearing lysine to glutamine mutations in the second and third lysine residues of the KISKK domain, pH 7.5, temperature not specified in the publication	Triticum aestivum
65	-	reduced flavodoxin	mutant bearing a lysine to glutamine mutation in the first lysine residue of the KISKK domain, pH 7.5, temperature not specified in the publication	Triticum aestivum
66.3	-	reduced flavodoxin	mutant bearing a lysine to glutamine mutation in the second lysine residue of the KISKK domain, pH 7.5, temperature not specified in the publication	Triticum aestivum
104	-	reduced flavodoxin	wild-type, pH 7.5, temperature not specified in the publication	Triticum aestivum
128.2	-	reduced flavodoxin	mutant bearing an N-terminal region with the full wheat KISKK domain, pH 7.5, temperature not specified in the publication	Triticum aestivum
139.4	-	reduced flavodoxin	mutant with an extended N-terminal region to include the EAxxPA motif of maize, pH 7.5, temperature not specified in the publication	Triticum aestivum

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
3700	-	ferricytochrome c	mutant bearing a truncated N-terminal domain beginning after the KISKK domain, pH 7.5, temperature not specified in the publication	Triticum aestivum
4520	-	ferricytochrome c	mutant bearing lysine to glutamine mutations in the second and third lysine residues of the KISKK domain, pH 7.5, temperature not specified in the publication	Triticum aestivum
12640	-	ferricytochrome c	mutant with an extended N-terminal region to include the EAxxPA motif of maize, pH 7.5, temperature not specified in the publication	Triticum aestivum
12830	-	ferricytochrome c	mutant bearing an N-terminal region with the full wheat KISKK domain, pH 7.5, temperature not specified in the publication	Triticum aestivum
18940	-	ferricytochrome c	mutant bearing a lysine to glutamine mutation in the second lysine residue of the KISKK domain, pH 7.5, temperature not specified in the publication	Triticum aestivum
31710	-	ferricytochrome c	mutant bearing a lysine to glutamine mutation in the first lysine residue of the KISKK domain, pH 7.5, temperature not specified in the publication	Triticum aestivum
33330	-	ferricytochrome c	wild-type, pH 7.5, temperature not specified in the publication	Triticum aestivum

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Release 2023.1 (January 2023)