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Literature summary for 1.18.1.2 extracted from

  • Takeda, K.; Sato, J.; Goto, K.; Fujita, T.; Watanabe, T.; Abo, M.; Yoshimura, E.; Nakagawa, J.; Abe, A.; Kawasaki, S.; Niimura, Y.
    Escherichia coli ferredoxin-NADP+ reductase and oxygen-insensitive nitroreductase are capable of functioning as ferric reductase and of driving the Fenton reaction (2010), Biometals, 23, 727-737.
    View publication on PubMed
Search for more literature for 1.18.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 cells Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0018
-
 NADPH in the presence of 0.15 mM Fe(III)-EDTA, in 50 mM sodium phosphate (pH 7.0), at 25°C Escherichia coli
0.00364
-
 Fe(III)-EDTA in the presence of 0.15 mM Fe(III)-EDTA, in the presence of 0.15 mM FAD, in 50 mM sodium phosphate (pH 7.0), at 25°C Escherichia coli
0.0396
-
 Fe(III)-EDTA in the presence of 0.15 mM Fe(III)-EDTA, in the absence of free flavin, in 50 mM sodium phosphate (pH 7.0), at 25°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 x * 28000, SDS-PAGE Escherichia coli
28981
-
 x * 28981, calculated from amino acid sequence Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P28861
-
-

Purification (Commentary)

Purification (Comment) Organism
butyl Toyopearl column chromatography and DEAE Sepharose column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricyanide + NADPH best substrate Escherichia coli 2 ferrocyanide + NADP+ + H+
-
 ?
cytochrome c + NADPH + H+ weakest substrate Escherichia coli ?
-
 ?
Fe(III)-deferoxamine + NADPH + H+
-
 Escherichia coli ?
-
 ?
Fe(III)-diethylenetriamine-N,N,N',N'',N''-pentaacetic acid + NADPH + H+ highest activity Escherichia coli Fe(II) + diethylenetriamine-N,N,N',N'',N''-pentaacetic acid + NADP+
-
 ?
Fe(III)-EDTA + NADPH + H+
-
 Escherichia coli Fe(II) + EDTA + NADP+
-
 ?
Fe(III)-ferrichrome + NADPH + H+
-
 Escherichia coli ?
-
 ?
Fe(III)-nitrilotriacetic acid + NADPH + H+
-
 Escherichia coli Fe(II) + nitrilotriacetic acid + NADP+
-
 ?
ferric citrate + NADPH + H+
-
 Escherichia coli ?
-
 ?
ferric enterobactin + NADPH + H+ lowest activity Escherichia coli ?
-
 ?
oxidized 2,6-dichlorophenolindophenol + NADPH + H+
-
 Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
? x * 28000, SDS-PAGE Escherichia coli
? x * 28981, calculated from amino acid sequence Escherichia coli

Synonyms

Synonyms Comment Organism
ferredoxin-NADP+ reductase
-
 Escherichia coli
ferric reductase
-
 Escherichia coli
FPR
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3
-
 Fe(III)-EDTA in the presence of 0.15 mM Fe(III)-EDTA, in the absence of free flavin, in 50 mM sodium phosphate (pH 7.0), at 25°C Escherichia coli
3.53
-
 Fe(III)-EDTA in the presence of 0.15 mM Fe(III)-EDTA, in the presence of 0.15 mM FAD, in 50 mM sodium phosphate (pH 7.0), at 25°C Escherichia coli

Cofactor

Cofactor Comment Organism Structure
FAD contains FAD, the Km for FAD is 0.0429 mM Escherichia coli
NADPH
-
 Escherichia coli

Expression

Organism Comment Expression
Escherichia coli the level of Fpr is significantly increased in Escherichia coli cultured in lower iron concentrations (0.017-12.2 ppm) up

General Information

General Information Comment Organism
metabolism the enzyme also drives the Fenton reaction Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
76.3
-
 Fe(III)-EDTA in the presence of 0.15 mM Fe(III)-EDTA, in the absence of free flavin, in 50 mM sodium phosphate (pH 7.0), at 25°C Escherichia coli
972
-
 Fe(III)-EDTA in the presence of 0.15 mM Fe(III)-EDTA, in the presence of 0.15 mM FAD, in 50 mM sodium phosphate (pH 7.0), at 25°C Escherichia coli