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Literature summary for 1.18.1.2 extracted from

  • Crobu, D.; Canevari, G.; Milani, M.; Pandini, V.; Vanoni, M.A.; Bolognesi, M.; Zanetti, G.; Aliverti, A.
    Plasmodium falciparum ferredoxin-NADP+ reductase His286 plays a dual role in NADP(H) binding and catalysis (2009), Biochemistry, 48, 9525-9533.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli Plasmodium falciparum

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Plasmodium falciparum

Protein Variants

Protein Variants Comment Organism
H286A kcat and kcat/Km are markedly reduced Plasmodium falciparum
H286K considerable decrease in kcat and kcat/Km, almost no reactivity with K3Fe(CN)6 Plasmodium falciparum
H286L kcat and kcat/Km are markedly reduced, anomalous reactivity with 2,6-dichlorophenolindophenol Plasmodium falciparum
H286Q up to 50% higher kcat than wild type, higher kcat/Km when 2,6-dichlorophenolindophenol serves as electron acceptor Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
2'-AMP when 2,6-dichlorophenolindophenol serves as electron acceptor Plasmodium falciparum
K3Fe(CN)6
-
Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
NADPH in an assay using 2,6-dichlorophenolindophenol as electron acceptor for mutant H286Q Plasmodium falciparum
0.036
-
NADPH in an assay using K3Fe(CN)6 as electron acceptor Plasmodium falciparum
0.057
-
NADPH in an assay using K3Fe(CN)6 as electron acceptor for mutant H286Q Plasmodium falciparum
0.071
-
NADPH in an assay using 2,6-dichlorophenolindophenol as electron acceptor Plasmodium falciparum
0.14
-
NADPH in an assay using 2,6-dichlorophenolindophenol as electron acceptor for mutant H286K Plasmodium falciparum
0.37
-
NADPH in an assay using K3Fe(CN)6 as electron acceptor for mutant H286L Plasmodium falciparum
0.4
-
NADPH in an assay using 2,6-dichlorophenolindophenol as electron acceptor for mutant H286A Plasmodium falciparum
0.52
-
NADPH in an assay using K3Fe(CN)6 as electron acceptor for mutant H286A Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum C6KT68
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADPH Plasmodium falciparum NADP+ + reduced 2,6-dichlorophenolindophenol
-
r
NADPH + K3Fe(CN)6 assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADPH Plasmodium falciparum NADP+ + reduced K3Fe(CN)6
-
r

Synonyms

Synonyms Comment Organism
ferredoxin-NADP+ reductase
-
Plasmodium falciparum
FNR
-
Plasmodium falciparum
PfFNR ferredoxin-NADP+ reductase of Plasmodium falciparum Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.8
-
NADPH in an assay using K3Fe(CN)6 as electron acceptor for mutant H286K Plasmodium falciparum
6
-
NADPH in an assay using 2,6-dichlorophenolindophenol as electron acceptor for mutant H286K Plasmodium falciparum
7.5
-
NADPH in an assay using K3Fe(CN)6 as electron acceptor for mutant H286L Plasmodium falciparum
17.5
-
NADPH in an assay using 2,6-dichlorophenolindophenol as electron acceptor for mutant H286A Plasmodium falciparum
44.5
-
NADPH in an assay using K3Fe(CN)6 as electron acceptor for mutant H286A Plasmodium falciparum
55
-
NADPH in an assay using 2,6-dichlorophenolindophenol as electron acceptor Plasmodium falciparum
70
-
NADPH in an assay using 2,6-dichlorophenolindophenol as electron acceptor for mutant H286Q Plasmodium falciparum
125
-
NADPH in an assay using K3Fe(CN)6 as electron acceptor Plasmodium falciparum
185
-
NADPH in an assay using K3Fe(CN)6 as electron acceptor for mutant H286Q Plasmodium falciparum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.024
-
2'-AMP when 2,6-dichlorophenolindophenol serves as electron acceptor Plasmodium falciparum
0.23
-
K3Fe(CN)6
-
Plasmodium falciparum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
20
-
NADPH in an assay using K3Fe(CN)6 as electron acceptor for mutant H286L Plasmodium falciparum
45
-
NADPH in an assay using 2,6-dichlorophenolindophenol as electron acceptor for mutant H286K Plasmodium falciparum
49
-
NADPH in an assay using 2,6-dichlorophenolindophenol as electron acceptor for mutant H286A Plasmodium falciparum
85
-
NADPH in an assay using K3Fe(CN)6 as electron acceptor for mutant H286A Plasmodium falciparum
750
-
NADPH in an assay using 2,6-dichlorophenolindophenol as electron acceptor Plasmodium falciparum
3150
-
NADPH in an assay using 2,6-dichlorophenolindophenol as electron acceptor for mutant H286Q Plasmodium falciparum
3300
-
NADPH in an assay using K3Fe(CN)6 as electron acceptor for mutant H286Q Plasmodium falciparum
3500
-
NADPH in an assay using K3Fe(CN)6 as electron acceptor Plasmodium falciparum