Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.18.1.2 extracted from

  • Lee, Y.; Yeom, J.; Kang, Y.S.; Kim, J.; Sung, J.S.; Jeon, C.O.; Park, W.
    Molecular characterization of fprB (ferredoxin-NADP+ reductase) in Pseudomonas putida KT2440 (2007), J. Microbiol. Biotechnol., 17, 1504-1512.
    View publication on PubMed
Search for more literature for 1.18.1.2

Activating Compound

Activating Compound Comment Organism Structure
NaCl 0.51 M and 0.85 M NaCl increases Fpr expression Pseudomonas putida

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
additional information induction of FprB expression is not affected by oxidative stress agents, such as paraquat, menadione, H2O2, and t-butyl hydroperoxide Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28600
-
 calculated from amino acid sequence Pseudomonas putida
29000
-
 SDS-PAGE, purified enzyme Pseudomonas putida
35000
-
 Sephacryl S-200 gel filtration, native enzyme Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida KT 2240
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography and Sephacryl S-300 gel filtration Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Fpr also catalyzes irreversible electron transfer (diaphorase activity), which drives the oxidation of NADPH in a wide variety of electron acceptors, such as viologens, quinines, complexed transition metals, and tetrazolium salts Pseudomonas putida ?
-
 ?
additional information Fpr also catalyzes irreversible electron transfer (diaphorase activity), which drives the oxidation of NADPH in a wide variety of electron acceptors, such as viologens, quinines, complexed transition metals, and tetrazolium salts Pseudomonas putida KT 2240 ?
-
 ?
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol diaphorase reaction Pseudomonas putida NADP+ + reduced 2,6-dichlorophenolindophenol
-
 ir
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol diaphorase reaction Pseudomonas putida KT 2240 NADP+ + reduced 2,6-dichlorophenolindophenol
-
 ir
reduced ferredoxin + NADP+
-
 Pseudomonas putida oxidized ferredoxin + NADPH
-
 r
reduced ferredoxin + NADP+
-
 Pseudomonas putida KT 2240 oxidized ferredoxin + NADPH
-
 r

Subunits

Subunits Comment Organism
monomer 1 * 35000, Sephacryl S-200 gel filtration, native enzyme Pseudomonas putida

Synonyms

Synonyms Comment Organism
ferredoxin-NADP+ reductase
-
 Pseudomonas putida
FprB
-
 Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Pseudomonas putida