Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Tuner(DE3)pLacI cells | Chlorobaculum tepidum |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method, using 0.1 M MES buffer pH 5.8 containing 16% (w/v) PEG 6K and 0.1 M ammonium sulfate as precipitant | Chlorobaculum tepidum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
2 * 45000, gel filtration | Chlorobaculum tepidum |
90000 | - |
gel filtration | Chlorobaculum tepidum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlorobaculum tepidum | - |
- |
- |
Chlorobaculum tepidum CT1512 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Matrex Red A column chromatography, DEAE-5PW column chromatography, hydroxyapatite column chromatography, and Superdex-200 gel filtration | Chlorobaculum tepidum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced ferredoxin + NAD(P)+ | - |
Chlorobaculum tepidum | oxidized ferredoxin + NAD(P)H | - |
r | |
reduced ferredoxin + NAD(P)+ | - |
Chlorobaculum tepidum CT1512 | oxidized ferredoxin + NAD(P)H | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 45000, gel filtration | Chlorobaculum tepidum |
Synonyms | Comment | Organism |
---|---|---|
Fd-NADP+ reductase | - |
Chlorobaculum tepidum |
Ferredoxin-NADP(+) reductase | - |
Chlorobaculum tepidum |
FNR | - |
Chlorobaculum tepidum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | - |
Chlorobaculum tepidum | |
NAD(P)+ | - |
Chlorobaculum tepidum |