Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, no posttranslational modification of the recombinant enzyme, the recombinant enzyme shows 26% of native enzyme activity | Chlamydomonas reinhardtii |
Protein Variants | Comment | Organism |
---|---|---|
K135L | site-directed mutagenesis, altered tertiary structure compared to the wild-type enzyme, no methylation at K83, 10% of native wild-type enzyme activity | Chlamydomonas reinhardtii |
K83L | site-directed mutagenesis, altered tertiary structure compared to the wild-type enzyme, no methylation at K83, 2-4% of native wild-type enzyme activity | Chlamydomonas reinhardtii |
K83L/K89L | site-directed mutagenesis, altered tertiary structure compared to the wild-type enzyme, no methylation at K83, 0.3% of native wild-type enzyme activity | Chlamydomonas reinhardtii |
K89L | site-directed mutagenesis, altered tertiary structure compared to the wild-type enzyme, no methylation at K83, 4% of native wild-type enzyme activity | Chlamydomonas reinhardtii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Chlamydomonas reinhardtii | |
0.0026 | - |
reduced ferredoxin | native enzyme, pH 8.0, 30°C | Chlamydomonas reinhardtii | |
0.0066 | - |
reduced ferredoxin | recombinant enzyme, pH 8.0, 30°C | Chlamydomonas reinhardtii | |
0.007 | - |
NADPH | native enzyme, pH 8.0, 30°C | Chlamydomonas reinhardtii | |
0.033 | - |
NADPH | recombinant enzyme, pH 8.0, 30°C | Chlamydomonas reinhardtii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36500 | - |
native enzyme, gel filtration | Chlamydomonas reinhardtii |
37500 | - |
native enzyme, native PAGE | Chlamydomonas reinhardtii |
38500 | - |
recombinant enzyme, gel filtration | Chlamydomonas reinhardtii |
42000 | - |
recombinant enzyme, native PAGE | Chlamydomonas reinhardtii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | the native enzyme is posttranslationally trimethylated at lysine residues K83, K89, and K135 inducing changes in the tertiary enzyme structure | Chlamydomonas reinhardtii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH | positively charged residues K83 and K89 are required for catalytic activity, methylation of K83, K89, and K135 is required for optimal enzyme activity | Chlamydomonas reinhardtii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Chlamydomonas reinhardtii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,6-dichlorophenolindophenol + NADPH + H+ | - |
Chlamydomonas reinhardtii | reduced 2,6-dichlorophenolindophenol + NADP+ | - |
r | |
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol | diaphorase activity | Chlamydomonas reinhardtii | NADP+ + reduced 2,6-dichlorophenolindophenol | - |
? | |
reduced ferredoxin + NADP+ | ferredoxin-dependent cytochrome c reduction, ferredoxin binding is independent of enzyme methylation status, positively charged residues K83 and K89 are required for activity | Chlamydomonas reinhardtii | oxidized ferredoxin + NADPH | - |
? | |
reduced ferredoxin + NADP+ | - |
Chlamydomonas reinhardtii | oxidized ferredoxin + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Chlamydomonas reinhardtii |
Synonyms | Comment | Organism |
---|---|---|
ferredoxin-NADP+-reductase | - |
Chlamydomonas reinhardtii |
ferredoxin-NADP-reductase | - |
Chlamydomonas reinhardtii |
FNR | - |
Chlamydomonas reinhardtii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Chlamydomonas reinhardtii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
35 | - |
2,6-dichlorophenolindophenol | native enzyme, pH 8.0, 30°C | Chlamydomonas reinhardtii | |
37 | - |
2,6-dichlorophenolindophenol | recombinant enzyme, pH 8.0, 30°C | Chlamydomonas reinhardtii | |
90 | - |
reduced ferredoxin | native enzyme, pH 8.0, 30°C | Chlamydomonas reinhardtii | |
174 | - |
reduced ferredoxin | recombinant enzyme, pH 8.0, 30°C | Chlamydomonas reinhardtii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Chlamydomonas reinhardtii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Chlamydomonas reinhardtii | |
NADPH | - |
Chlamydomonas reinhardtii |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Chlamydomonas reinhardtii | native and recombinant enzyme, isoelectric focusing | - |
5.8 |