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Literature summary for 1.18.1.1 extracted from
- Properties of rubredoxin reductase from the alkane-assimilating bacterium Acinetobacter calcoaceticus (1979), Z. Allg. Mikrobiol., 19, 695-704.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| FAD | addition of FAD increases activity | Acinetobacter calcoaceticus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 8-hydroxyquinoline | - |
Acinetobacter calcoaceticus | |
| AgNO3 | - |
Acinetobacter calcoaceticus | |
| BaCl2 | - |
Acinetobacter calcoaceticus | |
| CaCl2 | - |
Acinetobacter calcoaceticus | |
| deoxycholate | - |
Acinetobacter calcoaceticus | |
| HgCl2 | - |
Acinetobacter calcoaceticus | |
| PCMB | - |
Acinetobacter calcoaceticus | |
| ZnCl2 | - |
Acinetobacter calcoaceticus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0025 | - |
2,6-dichloroindophenol | - |
Acinetobacter calcoaceticus | |
| 0.055 | - |
Fe(CN)63- | - |
Acinetobacter calcoaceticus | |
| 0.11 | - |
NADH | reaction with 2,6-dichloroindophenol or Fe(CN)63- | Acinetobacter calcoaceticus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter calcoaceticus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADH + 2,6-dichloroindophenol | - |
Acinetobacter calcoaceticus | NAD+ + reduced 2,6-dichloroindophenol | - |
? | |
| NADH + Fe(CN)63- | - |
Acinetobacter calcoaceticus | NAD+ + Fe(CN)64- | - |
? | |
| NADH + oxidized rubredoxin | - |
Acinetobacter calcoaceticus | NAD+ + reduced rubredoxin | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
5 min, no inactivation in presence of FAD | Acinetobacter calcoaceticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.7 | - |
phosphate buffer | Acinetobacter calcoaceticus |
| 8.5 | - |
Tris-HCl buffer | Acinetobacter calcoaceticus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | prosthetic group | Acinetobacter calcoaceticus | |
| NADH | - |
Acinetobacter calcoaceticus | |
| NADPH | no significant reaction with | Acinetobacter calcoaceticus | |
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