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Literature summary for 1.17.99.4 extracted from

  • Wang, T.P.; Lampen, J.O.
    Uracil oxidase and the isolation of barbituric acid from uracil oxidation (1952), J. Biol. Chem., 194, 785-791.
    View publication on PubMed
Search for more literature for 1.17.99.4

Activating Compound

Activating Compound Comment Organism Structure
methylene blue required as electron acceptor Aerobic soil bacterium

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Aerobic soil bacterium 5737
-

Organism

Organism UniProt Comment Textmining
Aerobic soil bacterium
-
-
-
Aerobic soil bacterium U-1
-
-
-

Storage Stability

Storage Stability Organism
-20°C, 80% loss of activity in crude extracts within two months Aerobic soil bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-aminouracil + acceptor
-
 Aerobic soil bacterium ?
-
 ?
5-aminouracil + acceptor
-
 Aerobic soil bacterium U-1 ?
-
 ?
thymine + acceptor
-
 Aerobic soil bacterium 5-methylbarbiturate + reduced acceptor
-
 ?
thymine + acceptor
-
 Aerobic soil bacterium U-1 5-methylbarbiturate + reduced acceptor
-
 ?
uracil + acceptor barbiturate, isobarbiturate, 5-methylbarbiturate, 6-methyluracil, dihydrothymine, dihydrouracil, 2-thiouracil, 2-thiothymine, 2-thio-5-methyluracil and cytosine are no substrates Aerobic soil bacterium barbiturate + reduced acceptor
-
 ?
uracil + acceptor acceptor: 2,6-dichlorophenolindophenol or methylene blue act as artificial electron acceptors, O2 only in the presence of methylene blue Aerobic soil bacterium barbiturate + reduced acceptor
-
 ?
uracil + acceptor barbiturate, isobarbiturate, 5-methylbarbiturate, 6-methyluracil, dihydrothymine, dihydrouracil, 2-thiouracil, 2-thiothymine, 2-thio-5-methyluracil and cytosine are no substrates Aerobic soil bacterium U-1 barbiturate + reduced acceptor
-
 ?
uracil + acceptor acceptor: 2,6-dichlorophenolindophenol or methylene blue act as artificial electron acceptors, O2 only in the presence of methylene blue Aerobic soil bacterium U-1 barbiturate + reduced acceptor
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Aerobic soil bacterium

Cofactor

Cofactor Comment Organism Structure
additional information no oxidation in the presence of O2 without methylene blue as electron acceptor Aerobic soil bacterium