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Literature summary for 1.17.99.2 extracted from

  • Tataruch, M.; Heider, J.; Bryjak, J.; Nowak, P.; Knack, D.; Czerniak, A.; Liesiene, J.; Szaleniec, M.
    Suitability of the hydrocarbon-hydroxylating molybdenum-enzyme ethylbenzene dehydrogenase for industrial chiral alcohol production (2014), J. Biotechnol., 192, 400-409 .
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
ferrocenium with 3-phenyl-1-propene as cosubstrate, at pH 7.5 and 25°C Aromatoleum aromaticum
0.028
-
ferricyanide with ethylbenzene as cosubstrate, at pH 7.5 and 25°C Aromatoleum aromaticum
0.77
-
ferricyanide with 3-phenyl-1-propyne as cosubstrate, at pH 7.5 and 25°C Aromatoleum aromaticum
0.035
-
ferrocenium with 3-phenyl-1-propyne as cosubstrate, at pH 7.5 and 25°C Aromatoleum aromaticum
0.0098
-
ferrocenium with ethylbenzene as cosubstrate, at pH 7.5 and 25°C Aromatoleum aromaticum
0.362
-
ferricyanide with 3-phenyl-1-propene as cosubstrate, at pH 7.5 and 25°C Aromatoleum aromaticum

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Aromatoleum aromaticum
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ethylbenzene + H2O + acceptor Aromatoleum aromaticum
-
(S)-1-phenylethanol + reduced acceptor
-
?
ethylbenzene + H2O + acceptor Aromatoleum aromaticum EbN1
-
(S)-1-phenylethanol + reduced acceptor
-
?

Organism

Organism UniProt Comment Textmining
Aromatoleum aromaticum
-
-
-
Aromatoleum aromaticum EbN1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography Aromatoleum aromaticum

Storage Stability

Storage Stability Organism
4°C, partially purified enzyme, 6 days, almost no loss of activity Aromatoleum aromaticum
4°C, completely purified enzyme, 24 days, 78% loss of activity Aromatoleum aromaticum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethylbenzene + H2O + acceptor
-
Aromatoleum aromaticum (S)-1-phenylethanol + reduced acceptor
-
?
ethylbenzene + H2O + acceptor
-
Aromatoleum aromaticum EbN1 (S)-1-phenylethanol + reduced acceptor
-
?
2-ethylthiophene + ferricyanide
-
Aromatoleum aromaticum (S)-1-(2-thienyl)-ethanol + ferrocyanide
-
?
2-ethylthiophene + ferricyanide
-
Aromatoleum aromaticum EbN1 (S)-1-(2-thienyl)-ethanol + ferrocyanide
-
?
3-phenyl-1-propene + H2O + ferricyanide
-
Aromatoleum aromaticum ? + ferrocyanide
-
?
3-phenyl-1-propene + H2O + ferricyanide
-
Aromatoleum aromaticum EbN1 ? + ferrocyanide
-
?
3-phenyl-1-propene + H2O + ferrocenium
-
Aromatoleum aromaticum ? + ferrocene
-
?
3-phenyl-1-propene + H2O + ferrocenium
-
Aromatoleum aromaticum EbN1 ? + ferrocene
-
?
3-phenyl-1-propyne + H2O + ferricyanide
-
Aromatoleum aromaticum ? + ferrocyanide
-
?
3-phenyl-1-propyne + H2O + ferricyanide
-
Aromatoleum aromaticum EbN1 ? + ferrocyanide
-
?
3-phenyl-1-propyne + H2O + ferrocenium
-
Aromatoleum aromaticum ? + ferrocene
-
?
ethylbenzene + H2O + ferricyanide
-
Aromatoleum aromaticum (S)-1-phenylethanol + ferrocyanide
-
?
ethylbenzene + H2O + ferrocenium
-
Aromatoleum aromaticum (S)-1-phenylethanol + ferrocene
-
?

Synonyms

Synonyms Comment Organism
EBDH
-
Aromatoleum aromaticum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.17
-
ethylbenzene with ferricyanide as cosubstrate, at pH 7.5 and 25°C Aromatoleum aromaticum
0.64
-
3-phenyl-1-propene with ferricyanide as cosubstrate, at pH 7.5 and 25°C Aromatoleum aromaticum
0.46
-
3-phenyl-1-propene with ferrocenium as cosubstrate, at pH 7.5 and 25°C Aromatoleum aromaticum
0.29
-
3-phenyl-1-propyne with ferrocenium as cosubstrate, at pH 7.5 and 25°C Aromatoleum aromaticum
0.27
-
ethylbenzene with ferrocenium as cosubstrate, at pH 7.5 and 25°C Aromatoleum aromaticum
0.33
-
3-phenyl-1-propyne with ferricyanide as cosubstrate, at pH 7.5 and 25°C Aromatoleum aromaticum

Cofactor

Cofactor Comment Organism Structure
heme
-
Aromatoleum aromaticum
iron-sulfur centre
-
Aromatoleum aromaticum
bis(molybdopterin guanine dinucleotide)molybdenum
-
Aromatoleum aromaticum