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Literature summary for 1.17.99.2 extracted from

  • Szaleniec, M.; Hagel, C.; Menke, M.; Nowak, P.; Witko, M.; Heider, J.
    Kinetics and mechanism of oxygen-independent hydrocarbon hydroxylation by ethylbenzene dehydrogenase (2007), Biochemistry, 46, 7637-7646.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
O2 the enzyme is quickly inactivated in air in its reduced state Azoarcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Azoarcus sp.
0.045
-
ethylbenzene pH 7.5, 30°C Azoarcus sp.
0.0018
-
3-ethylpyridine pH 7.5, 30°C Azoarcus sp.
0.0023
-
2-ethylpyrrole pH 7.5, 30°C Azoarcus sp.
0.0052
-
2-Ethylphenol pH 7.5, 30°C Azoarcus sp.
0.0665
-
2-ethylthiophene pH 7.5, 30°C Azoarcus sp.
0.0093
-
3-Ethylphenol pH 7.5, 30°C Azoarcus sp.
0.0283
-
ferricenium pH 7.5, 30°C Azoarcus sp.
0.0405
-
4-Ethylphenol pH 7.5, 30°C Azoarcus sp.
0.0243
-
2-ethylaniline pH 7.5, 30°C Azoarcus sp.
0.0247
-
2-ethylfuran pH 7.5, 30°C Azoarcus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ a molybdenum/iron-sulfur/heme protein Azoarcus sp.
Molybdenum a molybdenum/iron-sulfur/heme protein Azoarcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
155000
-
-
Azoarcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ethylbenzene + H2O + acceptor Azoarcus sp.
-
(S)-1-phenylethanol + reduced acceptor
-
?
ethylbenzene + H2O + acceptor Azoarcus sp. EbN1
-
(S)-1-phenylethanol + reduced acceptor
-
?

Organism

Organism UniProt Comment Textmining
Azoarcus sp.
-
i.e. Aromatoleum aromaticum
-
Azoarcus sp. EbN1
-
i.e. Aromatoleum aromaticum
-

Oxidation Stability

Oxidation Stability Organism
the enzyme is quickly inactivated in air in its reduced state Azoarcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity, product identification by GC- and LC-MS, overview Azoarcus sp. ?
-
?
additional information substrate specificity, product identification by GC- and LC-MS, overview Azoarcus sp. EbN1 ?
-
?
ethylbenzene + H2O + acceptor
-
Azoarcus sp. (S)-1-phenylethanol + reduced acceptor
-
?
ethylbenzene + H2O + acceptor
-
Azoarcus sp. EbN1 (S)-1-phenylethanol + reduced acceptor
-
?
1,4-diethylbenzene + H2O + ferricenium
-
Azoarcus sp. 1-(4-ethylphenyl)ethanol + 1,1'-(1,4-phenylene)diethanol + ferrocenium
-
?
1,4-diethylbenzene + H2O + ferricenium
-
Azoarcus sp. EbN1 1-(4-ethylphenyl)ethanol + 1,1'-(1,4-phenylene)diethanol + ferrocenium
-
?
4-ethylbiphenyl + H2O + ferricenium
-
Azoarcus sp. 1-(1,1'-biphenyl-4-yl)ethanol + reduced ferrocenium
-
?
4-ethylbiphenyl + H2O + ferricenium
-
Azoarcus sp. EbN1 1-(1,1'-biphenyl-4-yl)ethanol + reduced ferrocenium
-
?
2-ethylaniline + H2O + ferricenium
-
Azoarcus sp. ?
-
?
2-ethylaniline + H2O + ferricenium
-
Azoarcus sp. EbN1 ?
-
?
2-ethylfuran + H2O + ferricenium
-
Azoarcus sp. 1-(2-furyl)ethanol + ferrocenium
-
?
2-ethylfuran + H2O + ferricenium
-
Azoarcus sp. ?
-
?
2-ethylphenol + H2O + ferricenium
-
Azoarcus sp. 2-(1-hydroxyethyl)phenol + ferrocenium
-
?
2-ethylpyrrole + H2O + ferricenium
-
Azoarcus sp. ?
-
?
2-ethylthiophene + H2O + ferricenium
-
Azoarcus sp. 1-thien-2-ylethanol + ferrocenium
-
?
2-ethylthiophene + H2O + ferricenium
-
Azoarcus sp. ?
-
?
3-ethylphenol + H2O + ferricenium
-
Azoarcus sp. 3-(1-hydroxyethyl)phenol + ferrocenium
-
?
3-ethylpyridine + H2O + ferricenium
-
Azoarcus sp. ?
-
?
4-ethylanisole + H2O + ferricenium
-
Azoarcus sp. 1-(4-methoxyphenyl)ethanol + ferrocenium
-
?
4-ethylphenol + H2O + ferricenium best substrate Azoarcus sp. 4-(1-hydroxyethyl)phenol + ferrocenium
-
?
4-ethyltoluene + H2O + ferricenium
-
Azoarcus sp. 1-(4-methylphenyl)ethanol + ferrocenium
-
?
4-propylphenol + H2O + ferricenium
-
Azoarcus sp. 4-(1-hydroxypropyl)phenol + ferrocenium
-
?
ethylbenzene + H2O + ferricenium EBDH catalyzes the oxygen-independent, stereospecific hydroxylation of ethylbenzene to (S)-1-phenylethanol, the direct anaerobic oxidation of a nonactivated hydrocarbon Azoarcus sp. (S)-1-phenylethanol + ferrocenium
-
?
ethylidenecyclohexane + H2O + ferricenium
-
Azoarcus sp. ?
-
?
propylbenzene + H2O + ferricenium
-
Azoarcus sp. (S)-1-phenylpropanol + ferrocenium
-
?

Subunits

Subunits Comment Organism
trimer
-
Azoarcus sp.
More quantitative structure-activity relationship modeling Azoarcus sp.

Synonyms

Synonyms Comment Organism
ethylbenzene dehydrogenase
-
Azoarcus sp.
EBDH
-
Azoarcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Azoarcus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information relative kcat values Azoarcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Azoarcus sp.

Cofactor

Cofactor Comment Organism Structure
heme a molybdenum/iron-sulfur/heme protein Azoarcus sp.