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Literature summary for 1.17.99.2 extracted from

  • Johnson, H.A.; Pelletier, D.A.; Spormann, A.M.
    Isolation and characterisation of anaerobic ethylbenzene dehydrogenase, a novel Mo-Fe-S enzyme (2001), J. Bacteriol., 183, 4536-4542.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
in Escherichia coli DH5alpha Azoarcus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Azoarcus sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
iron sulfur cluster located in beta-subunit Azoarcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
calculated from DNA-sequence Azoarcus sp.
70000
-
gel filtration of purified enzyme Azoarcus sp.
100000
-
alpha,beta,gamma 1 * 100000 + 1 * 35000 + 1 * 25000, SDS-PAGE, 1 * 104226 + 1 * 39643 + 1 * 23061, calculated from DNA-sequence Azoarcus sp.
35000
-
alpha,beta,gamma 1 * 100000 + 1 * 35000 + 1 * 25000, SDS-PAGE, 1 * 104226 + 1 * 39643 + 1 * 23061, calculated from DNA-sequence Azoarcus sp.
25000
-
alpha,beta,gamma 1 * 100000 + 1 * 35000 + 1 * 25000, SDS-PAGE, 1 * 104226 + 1 * 39643 + 1 * 23061, calculated from DNA-sequence Azoarcus sp.
545000
-
native PAGE Azoarcus sp.
104226
-
alpha,beta,gamma 1 * 100000 + 1 * 35000 + 1 * 25000, SDS-PAGE, 1 * 104226 + 1 * 39643 + 1 * 23061, calculated from DNA-sequence Azoarcus sp.
39643
-
alpha,beta,gamma 1 * 100000 + 1 * 35000 + 1 * 25000, SDS-PAGE, 1 * 104226 + 1 * 39643 + 1 * 23061, calculated from DNA-sequence Azoarcus sp.
23061
-
alpha,beta,gamma 1 * 100000 + 1 * 35000 + 1 * 25000, SDS-PAGE, 1 * 104226 + 1 * 39643 + 1 * 23061, calculated from DNA-sequence Azoarcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ethylbenzene + H2O + acceptor Azoarcus sp. anaerobic degradation of ethylbenzene in bacteria (S)-1-phenylethanol + reduced acceptor
-
?
ethylbenzene + H2O + acceptor Azoarcus sp. EB1 anaerobic degradation of ethylbenzene in bacteria (S)-1-phenylethanol + reduced acceptor
-
?

Organism

Organism UniProt Comment Textmining
Azoarcus sp.
-
EB1
-
Azoarcus sp. EB1
-
EB1
-

Purification (Commentary)

Purification (Comment) Organism
-
Azoarcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethylbenzene + H2O + molybdopterin
-
Azoarcus sp. (S)-1-phenylethanol + molybdopterin (reduced form)
-
?
ethylbenzene + H2O + molybdopterin
-
Azoarcus sp. EB1 (S)-1-phenylethanol + molybdopterin (reduced form)
-
?
4-fluoro-ethylbenzene + molybdopterin
-
Azoarcus sp. ?
-
?
4-fluoro-ethylbenzene + molybdopterin
-
Azoarcus sp. EB1 ?
-
?
3-methyl-2-pentene + molybdopterin
-
Azoarcus sp. ?
-
?
3-methyl-2-pentene + molybdopterin
-
Azoarcus sp. EB1 ?
-
?
ethylidenecyclohexane + molybdopterin
-
Azoarcus sp. ?
-
?
ethylidenecyclohexane + molybdopterin
-
Azoarcus sp. EB1 ?
-
?
ethylbenzene + H2O + acceptor anaerobic degradation of ethylbenzene in bacteria Azoarcus sp. (S)-1-phenylethanol + reduced acceptor
-
?
ethylbenzene + H2O + acceptor anaerobic degradation of ethylbenzene in bacteria Azoarcus sp. EB1 (S)-1-phenylethanol + reduced acceptor
-
?

Subunits

Subunits Comment Organism
trimer alpha,beta,gamma 1 * 100000 + 1 * 35000 + 1 * 25000, SDS-PAGE, 1 * 104226 + 1 * 39643 + 1 * 23061, calculated from DNA-sequence Azoarcus sp.

Cofactor

Cofactor Comment Organism Structure
molybdopterin
-
Azoarcus sp.