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Literature summary for 1.17.99.11 extracted from

  • Yang, F.; Chen, Y.; Tang, S.; Yu, C.; Wang, P.; Ismail, W.; Wang, C.; Ding, J.; Yang, C.; Yang, C.; Chiang, Y.
    Integrated multi-omics analyses reveal the biochemical mechanisms and phylogenetic relevance of anaerobic androgen biodegradation in the environment (2016), ISME J., 10, 1967-1983 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
analysis the substrate preference of AtcABC enables the application of atcABC genes as molecular biomarkers to assess anaerobic androgen biodegradation in the environment Steroidobacter denitrificans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.052
-
1-testosterone pH 7.5, 30°C Steroidobacter denitrificans

Metals/Ions

Metals/Ions Comment Organism Structure
Mo2+ a molybdenum enzyme Steroidobacter denitrificans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-testosterone + H2O + acceptor Steroidobacter denitrificans the enzyme is involved in the anaerobic degradation of steroid A-ring 1,17-dihydroxy-androstan-3-one + reduced acceptor
-
?
1-testosterone + H2O + acceptor Steroidobacter denitrificans DSM 18526 the enzyme is involved in the anaerobic degradation of steroid A-ring 1,17-dihydroxy-androstan-3-one + reduced acceptor
-
?

Organism

Organism UniProt Comment Textmining
Steroidobacter denitrificans A0A127F6U7 AND A0A127F985 AND A0A127F9C1 A0A127F6U7: AtcA, A0A127F985: AtcB,A0A127F9C1: AtcC
-
Steroidobacter denitrificans DSM 18526 A0A127F6U7 AND A0A127F985 AND A0A127F9C1 A0A127F6U7: AtcA, A0A127F985: AtcB,A0A127F9C1: AtcC
-

Purification (Commentary)

Purification (Comment) Organism
-
Steroidobacter denitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-dehydrotestosterone + H2O + 2,6-dichlorophenolindophenol at about 10% of the activity as compared to 1-testosterone Steroidobacter denitrificans ? + reduced 2,6-dichlorophenolindophenol
-
?
1-dehydrotestosterone + H2O + 2,6-dichlorophenolindophenol at about 10% of the activity as compared to 1-testosterone Steroidobacter denitrificans DSM 18526 ? + reduced 2,6-dichlorophenolindophenol
-
?
1-testosterone + H2O + 2,6-dichlorophenolindophenol
-
Steroidobacter denitrificans 1,17-dihydroxy-androstan-3-one + reduced 2,6-dichlorophenolindophenol
-
?
1-testosterone + H2O + 2,6-dichlorophenolindophenol
-
Steroidobacter denitrificans DSM 18526 1,17-dihydroxy-androstan-3-one + reduced 2,6-dichlorophenolindophenol
-
?
1-testosterone + H2O + acceptor the enzyme is involved in the anaerobic degradation of steroid A-ring Steroidobacter denitrificans 1,17-dihydroxy-androstan-3-one + reduced acceptor
-
?
1-testosterone + H2O + acceptor the enzyme is involved in the anaerobic degradation of steroid A-ring Steroidobacter denitrificans DSM 18526 1,17-dihydroxy-androstan-3-one + reduced acceptor
-
?
2-octenal + 2,6-dichlorophenolindophenol at about 20% of the activity as compared to 1-testosterone Steroidobacter denitrificans ? + reduced 2,6-dichlorophenolindophenol
-
?
2-octenal + 2,6-dichlorophenolindophenol at about 20% of the activity as compared to 1-testosterone Steroidobacter denitrificans DSM 18526 ? + reduced 2,6-dichlorophenolindophenol
-
?
a 3-oxo-DELTA1-steroid + H2O + acceptor overall reaction. The enzyme is specific to 3-oxo-DELTA1-steroids such as androsta-1-ene-3,17-dione and DELTA1-dihydrotestosterone and does not act on 3-oxo-DELTA4-steroids Steroidobacter denitrificans a steroid 1,3-dione + reduced acceptor
-
?
a 3-oxo-DELTA1-steroid + H2O + acceptor overall reaction. The enzyme is specific to 3-oxo-DELTA1-steroids such as androsta-1-ene-3,17-dione and DELTA1-dihydrotestosterone and does not act on 3-oxo-DELTA4-steroids Steroidobacter denitrificans DSM 18526 a steroid 1,3-dione + reduced acceptor
-
?
androsta-1-ene-3,17-dione + H2O + 2,6-dichlorophenolindophenol at about 70% of the activity as compared to 1-testosterone Steroidobacter denitrificans ? + reduced 2,6-dichlorophenolindophenol
-
?

Subunits

Subunits Comment Organism
? x * 85000 (large protein AtcA) + x * 30000 (medium protein AtcB) + x * 18000 (small protein AtcC), SDS-PAGE Steroidobacter denitrificans

Synonyms

Synonyms Comment Organism
1-testosterone hydratase/dehydrogenase
-
Steroidobacter denitrificans
ACG33_03370 gene name, small protein AtcC Steroidobacter denitrificans
ACG33_03375 gene name, medium protein AtcB Steroidobacter denitrificans
ACG33_03390 gene name, large protein AtcA Steroidobacter denitrificans
atcABC
-
Steroidobacter denitrificans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Steroidobacter denitrificans

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8 pH 5.5: about 30% of maximal activity, pH 8-0: about 50% of maximal activity Steroidobacter denitrificans

General Information

General Information Comment Organism
physiological function the enzyme is involved in the anaerobic degradation of steroid A-ring Steroidobacter denitrificans