BRENDA - Enzyme Database
show all sequences of 1.17.98.2

BciD is a radical S-adenosyl-L-methionine (SAM) enzyme that completes bacteriochlorophyllide e biosynthesis by oxidizing a methyl group into a formyl group at C-7

Thweatt, J.; Ferlez, B.; Golbeck, J.; Bryant, D.; J. Biol. Chem. 292, 1361-1373 (2017)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Chlorobaculum limnaeum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + a 7-(hydroxymethyl)bacteriochlorophyllide c + H2O
Chlorobaculum limnaeum
-
a 7-(dihydroxymethyl)bacteriochlorophyllide c + 5'-deoxyadenosine + L-methionine
-
-
?
S-adenosyl-L-methionine + a 7-(hydroxymethyl)bacteriochlorophyllide c + H2O
Chlorobaculum limnaeum DSMZ 1677
-
a 7-(dihydroxymethyl)bacteriochlorophyllide c + 5'-deoxyadenosine + L-methionine
-
-
?
S-adenosyl-L-methionine + a bacteriochlorophyllide c + H2O
Chlorobaculum limnaeum
-
a 7-(hydroxymethyl)bacteriochlorophyllide c + 5'-deoxyadenosine + L-methionine
-
-
?
S-adenosyl-L-methionine + a bacteriochlorophyllide c + H2O
Chlorobaculum limnaeum
-
a bacteriochlorophyllide e + 5'-deoxyadenosine + L-methionine
-
-
?
S-adenosyl-L-methionine + a bacteriochlorophyllide c + H2O
Chlorobaculum limnaeum DSMZ 1677
-
a 7-(hydroxymethyl)bacteriochlorophyllide c + 5'-deoxyadenosine + L-methionine
-
-
?
S-adenosyl-L-methionine + a bacteriochlorophyllide c + H2O
Chlorobaculum limnaeum DSMZ 1677
-
a bacteriochlorophyllide e + 5'-deoxyadenosine + L-methionine
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Chlorobaculum limnaeum
N0DKX5
-
-
Chlorobaculum limnaeum DSMZ 1677
N0DKX5
-
-
Purification (Commentary)
Commentary
Organism
Ni-NTA column chromatography, and Superdex 200 gel filtration
Chlorobaculum limnaeum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + a 7-(hydroxymethyl)bacteriochlorophyllide c + H2O
-
745366
Chlorobaculum limnaeum
a 7-(dihydroxymethyl)bacteriochlorophyllide c + 5'-deoxyadenosine + L-methionine
-
-
-
?
S-adenosyl-L-methionine + a 7-(hydroxymethyl)bacteriochlorophyllide c + H2O
-
745366
Chlorobaculum limnaeum DSMZ 1677
a 7-(dihydroxymethyl)bacteriochlorophyllide c + 5'-deoxyadenosine + L-methionine
-
-
-
?
S-adenosyl-L-methionine + a bacteriochlorophyllide c + H2O
-
745366
Chlorobaculum limnaeum
a 7-(hydroxymethyl)bacteriochlorophyllide c + 5'-deoxyadenosine + L-methionine
-
-
-
?
S-adenosyl-L-methionine + a bacteriochlorophyllide c + H2O
-
745366
Chlorobaculum limnaeum DSMZ 1677
a 7-(hydroxymethyl)bacteriochlorophyllide c + 5'-deoxyadenosine + L-methionine
-
-
-
?
S-adenosyl-L-methionine + a bacteriochlorophyllide c + H2O
-
745366
Chlorobaculum limnaeum
a bacteriochlorophyllide e + 5'-deoxyadenosine + L-methionine
-
-
-
?
S-adenosyl-L-methionine + a bacteriochlorophyllide c + H2O
-
745366
Chlorobaculum limnaeum DSMZ 1677
a bacteriochlorophyllide e + 5'-deoxyadenosine + L-methionine
-
-
-
?
S-adenosyl-L-methionine + a bacteriochlorophyllide d + H2O
-
745366
Chlorobaculum limnaeum
a bacteriochlorophyllide f + 5'-deoxyadenosine + L-methionine
-
-
-
?
S-adenosyl-L-methionine + a bacteriochlorophyllide d + H2O
-
745366
Chlorobaculum limnaeum DSMZ 1677
a bacteriochlorophyllide f + 5'-deoxyadenosine + L-methionine
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 44000, SDS-PAGE
Chlorobaculum limnaeum
Cofactor
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Chlorobaculum limnaeum
[4Fe-4S]-center
-
Chlorobaculum limnaeum
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Chlorobaculum limnaeum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Chlorobaculum limnaeum
[4Fe-4S]-center
-
Chlorobaculum limnaeum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + a 7-(hydroxymethyl)bacteriochlorophyllide c + H2O
Chlorobaculum limnaeum
-
a 7-(dihydroxymethyl)bacteriochlorophyllide c + 5'-deoxyadenosine + L-methionine
-
-
?
S-adenosyl-L-methionine + a 7-(hydroxymethyl)bacteriochlorophyllide c + H2O
Chlorobaculum limnaeum DSMZ 1677
-
a 7-(dihydroxymethyl)bacteriochlorophyllide c + 5'-deoxyadenosine + L-methionine
-
-
?
S-adenosyl-L-methionine + a bacteriochlorophyllide c + H2O
Chlorobaculum limnaeum
-
a 7-(hydroxymethyl)bacteriochlorophyllide c + 5'-deoxyadenosine + L-methionine
-
-
?
S-adenosyl-L-methionine + a bacteriochlorophyllide c + H2O
Chlorobaculum limnaeum
-
a bacteriochlorophyllide e + 5'-deoxyadenosine + L-methionine
-
-
?
S-adenosyl-L-methionine + a bacteriochlorophyllide c + H2O
Chlorobaculum limnaeum DSMZ 1677
-
a 7-(hydroxymethyl)bacteriochlorophyllide c + 5'-deoxyadenosine + L-methionine
-
-
?
S-adenosyl-L-methionine + a bacteriochlorophyllide c + H2O
Chlorobaculum limnaeum DSMZ 1677
-
a bacteriochlorophyllide e + 5'-deoxyadenosine + L-methionine
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography, and Superdex 200 gel filtration
Chlorobaculum limnaeum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + a 7-(hydroxymethyl)bacteriochlorophyllide c + H2O
-
745366
Chlorobaculum limnaeum
a 7-(dihydroxymethyl)bacteriochlorophyllide c + 5'-deoxyadenosine + L-methionine
-
-
-
?
S-adenosyl-L-methionine + a 7-(hydroxymethyl)bacteriochlorophyllide c + H2O
-
745366
Chlorobaculum limnaeum DSMZ 1677
a 7-(dihydroxymethyl)bacteriochlorophyllide c + 5'-deoxyadenosine + L-methionine
-
-
-
?
S-adenosyl-L-methionine + a bacteriochlorophyllide c + H2O
-
745366
Chlorobaculum limnaeum
a 7-(hydroxymethyl)bacteriochlorophyllide c + 5'-deoxyadenosine + L-methionine
-
-
-
?
S-adenosyl-L-methionine + a bacteriochlorophyllide c + H2O
-
745366
Chlorobaculum limnaeum DSMZ 1677
a 7-(hydroxymethyl)bacteriochlorophyllide c + 5'-deoxyadenosine + L-methionine
-
-
-
?
S-adenosyl-L-methionine + a bacteriochlorophyllide c + H2O
-
745366
Chlorobaculum limnaeum
a bacteriochlorophyllide e + 5'-deoxyadenosine + L-methionine
-
-
-
?
S-adenosyl-L-methionine + a bacteriochlorophyllide c + H2O
-
745366
Chlorobaculum limnaeum DSMZ 1677
a bacteriochlorophyllide e + 5'-deoxyadenosine + L-methionine
-
-
-
?
S-adenosyl-L-methionine + a bacteriochlorophyllide d + H2O
-
745366
Chlorobaculum limnaeum
a bacteriochlorophyllide f + 5'-deoxyadenosine + L-methionine
-
-
-
?
S-adenosyl-L-methionine + a bacteriochlorophyllide d + H2O
-
745366
Chlorobaculum limnaeum DSMZ 1677
a bacteriochlorophyllide f + 5'-deoxyadenosine + L-methionine
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 44000, SDS-PAGE
Chlorobaculum limnaeum
Other publictions for EC 1.17.98.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745366
Thweatt
BciD is a radical S-adenosyl- ...
Chlorobaculum limnaeum, Chlorobaculum limnaeum DSMZ 1677
J. Biol. Chem.
292
1361-1373
2017
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2
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1
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745604
Mizoguchi
-
Inactivation of bciD and bchU ...
Chlorobaculum limnaeum, Chlorobaculum limnaeum RK-j-1
J. Photochem. Photobiol. A
313
52-59
2015
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739252
Mizoguchi
Isolation and characterization ...
Chlorobaculum limnaeum
Photosyn. Res.
121
3-12
2014
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745982
Mizoguchi
Isolation and characterizatio ...
Chlorobaculum limnaeum, Chlorobaculum limnaeum RK-j-1
Photosyn. Res.
121
3-12
2014
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6
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3
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739460
Harada
Specific gene bciD for C7-meth ...
Chlorobaculum limnaeum
PLoS ONE
8
e60026
2013
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