Any feedback?
Literature summary for 1.17.9.1 extracted from
- p-Cresol methylhydroxylase: alteration of the structure of the flavoprotein subunit upon its binding to the cytochrome subunit (2005), Biochemistry, 44, 2963-2973.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Pseudomonas putida |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| native enzyme and alpha subunit, sitting drop vapor diffusion method | Pseudomonas putida |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 9200 | - |
alpha2beta2, 2 * 58700 + 2 * 9200, c-type cytochrome subunit PchC (beta) and two flavoprotein subunits PchF (alpha) | Pseudomonas putida |
| 58700 | - |
alpha2beta2, 2 * 58700 + 2 * 9200, c-type cytochrome subunit PchC (beta) and two flavoprotein subunits PchF (alpha) | Pseudomonas putida |
| 136000 | - |
- |
Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-cresol + acceptor + H2O | Pseudomonas putida | via intermediate 4-hydroxybenzylalcohol, degradation of toxic 4-cresol | 4-hydroxybenzaldehyde + reduced acceptor | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | P09788 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-cresol + acceptor + H2O | via intermediate 4-hydroxybenzylalcohol | Pseudomonas putida | 4-hydroxybenzaldehyde + reduced acceptor | - |
? | |
| 4-cresol + acceptor + H2O | via intermediate 4-hydroxybenzylalcohol, degradation of toxic 4-cresol | Pseudomonas putida | 4-hydroxybenzaldehyde + reduced acceptor | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | alpha2beta2, 2 * 58700 + 2 * 9200, c-type cytochrome subunit PchC (beta) and two flavoprotein subunits PchF (alpha) | Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Pseudomonas putida | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
