Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-(pyridin-3-yl)ethyl diphosphate | i.e. BPH-1027 | Aquifex aeolicus | |
BPH-1029 | - |
Aquifex aeolicus | |
BPH-293 | - |
Aquifex aeolicus | |
Imidazol | - |
Aquifex aeolicus | |
additional information | pyridine inhibitors of IspH directly bind to the unique fourth Fe in the 4Fe-4S cluster, spectral binding structure analysis and inhibitor design, overview | Aquifex aeolicus | |
N-methylpyridinium | - |
Aquifex aeolicus | |
pyridin-2-ylmethyl diphosphate | i.e. BPH-1029 | Aquifex aeolicus | |
pyridin-3-ylmethyl diphosphate | i.e. BPH-293 | Aquifex aeolicus | |
pyridin-4-ylmethyl trihydrogen diphosphate | i.e. BPH-1030 | Aquifex aeolicus | |
pyridine-N-oxide | - |
Aquifex aeolicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | IspH is a 4Fe-4S protein | Aquifex aeolicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H + H+ | Aquifex aeolicus | formation of a mixture of isopentenyl diphosphate and dimethylallyl diphosphate in a ratio 1:5 | dimethylallyl diphosphate + NAD(P)+ + H2O | - |
r | |
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H + H+ | Aquifex aeolicus | formation of a mixture of isopentenyl diphosphate and dimethylallyl diphosphate in a ratio 1:5 | isopentenyl diphosphate + NAD(P)+ + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | - |
gene ispH or lytB | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H + H+ | formation of a mixture of isopentenyl diphosphate and dimethylallyl diphosphate in a ratio 1:5 | Aquifex aeolicus | dimethylallyl diphosphate + NAD(P)+ + H2O | - |
r | |
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H + H+ | formation of a mixture of isopentenyl diphosphate and dimethylallyl diphosphate in a ratio 1:5 | Aquifex aeolicus | isopentenyl diphosphate + NAD(P)+ + H2O | - |
r | |
additional information | the IspH-catalyzed reaction involves formation of organometallic species, containing Fe-C bonds | Aquifex aeolicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HMBPP reductase | - |
Aquifex aeolicus |
ispH | - |
Aquifex aeolicus |
LytB | - |
Aquifex aeolicus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0091 | - |
pH not specified in the publication, temperature not specified in the publication | Aquifex aeolicus | 2-(pyridin-3-yl)ethyl diphosphate | |
0.149 | - |
pH not specified in the publication, temperature not specified in the publication | Aquifex aeolicus | pyridin-4-ylmethyl trihydrogen diphosphate | |
1.2 | - |
pH not specified in the publication, temperature not specified in the publication | Aquifex aeolicus | pyridin-2-ylmethyl diphosphate |
General Information | Comment | Organism |
---|---|---|
physiological function | IspH is a 4Fe-4S protein that carries out an essential reduction step in isoprenoid biosynthesis | Aquifex aeolicus |