Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.17.7.4 extracted from

  • Wang, W.; Li, J.; Wang, K.; Smirnova, T.I.; Oldfield, E.
    Pyridine inhibitor binding to the 4Fe-4S protein A. aeolicus IspH (LytB): a HYSCORE Investigation (2011), J. Am. Chem. Soc., 133, 6525-6528.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
2-(pyridin-3-yl)ethyl diphosphate i.e. BPH-1027 Aquifex aeolicus
BPH-1029
-
Aquifex aeolicus
BPH-293
-
Aquifex aeolicus
Imidazol
-
Aquifex aeolicus
additional information pyridine inhibitors of IspH directly bind to the unique fourth Fe in the 4Fe-4S cluster, spectral binding structure analysis and inhibitor design, overview Aquifex aeolicus
N-methylpyridinium
-
Aquifex aeolicus
pyridin-2-ylmethyl diphosphate i.e. BPH-1029 Aquifex aeolicus
pyridin-3-ylmethyl diphosphate i.e. BPH-293 Aquifex aeolicus
pyridin-4-ylmethyl trihydrogen diphosphate i.e. BPH-1030 Aquifex aeolicus
pyridine-N-oxide
-
Aquifex aeolicus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ IspH is a 4Fe-4S protein Aquifex aeolicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H + H+ Aquifex aeolicus formation of a mixture of isopentenyl diphosphate and dimethylallyl diphosphate in a ratio 1:5 dimethylallyl diphosphate + NAD(P)+ + H2O
-
r
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H + H+ Aquifex aeolicus formation of a mixture of isopentenyl diphosphate and dimethylallyl diphosphate in a ratio 1:5 isopentenyl diphosphate + NAD(P)+ + H2O
-
r

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus
-
gene ispH or lytB
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H + H+ formation of a mixture of isopentenyl diphosphate and dimethylallyl diphosphate in a ratio 1:5 Aquifex aeolicus dimethylallyl diphosphate + NAD(P)+ + H2O
-
r
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H + H+ formation of a mixture of isopentenyl diphosphate and dimethylallyl diphosphate in a ratio 1:5 Aquifex aeolicus isopentenyl diphosphate + NAD(P)+ + H2O
-
r
additional information the IspH-catalyzed reaction involves formation of organometallic species, containing Fe-C bonds Aquifex aeolicus ?
-
?

Synonyms

Synonyms Comment Organism
HMBPP reductase
-
Aquifex aeolicus
ispH
-
Aquifex aeolicus
LytB
-
Aquifex aeolicus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0091
-
pH not specified in the publication, temperature not specified in the publication Aquifex aeolicus 2-(pyridin-3-yl)ethyl diphosphate
0.149
-
pH not specified in the publication, temperature not specified in the publication Aquifex aeolicus pyridin-4-ylmethyl trihydrogen diphosphate
1.2
-
pH not specified in the publication, temperature not specified in the publication Aquifex aeolicus pyridin-2-ylmethyl diphosphate

General Information

General Information Comment Organism
physiological function IspH is a 4Fe-4S protein that carries out an essential reduction step in isoprenoid biosynthesis Aquifex aeolicus