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Literature summary for 1.17.7.2 extracted from

  • Wang, X.; Liu, L.
    Crystal structure and catalytic mechanism of 7-hydroxymethyl chlorophyll a reductase (2016), J. Biol. Chem., 291, 13349-13359 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.17.7.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.2 M MgCl2, 0.1 M Tris-HCl, pH 8.5, 25% (w/v) polyethylene glycol 3350, 3% (v/v) ethylene glycol Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
D237A inactive Arabidopsis thaliana
E262A the mutant loses its catalytic activity, probably for its impaired FAD binding capacity Arabidopsis thaliana
H417A the mutant formes inclusion bodies during expression, and hence its activity is not measureable Arabidopsis thaliana
H417N inactive Arabidopsis thaliana
H417Y inactive Arabidopsis thaliana
Q332A the mutant aggregates and its activity is not measureable Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
195000
-
 gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
71-hydroxychlorophyll a + reduced ferredoxin + H+ Arabidopsis thaliana
-
 chlorophyll a + oxidized ferredoxin + H2O
-
 ir

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q8GS60
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 200 gel filtration Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
71-hydroxychlorophyll a + reduced ferredoxin + H+
-
 Arabidopsis thaliana chlorophyll a + oxidized ferredoxin + H2O
-
 ir

Subunits

Subunits Comment Organism
trimer 3 * 49000, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
HCAR
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Arabidopsis thaliana
[4Fe-4S]-center
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
metabolism the enzyme is required for the degradation of light-harvesting complexes and is necessary for efficient photosynthesis by balancing the chlorophyll a/b ratio Arabidopsis thaliana