Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermosynechococcus vestitus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
[4Fe-4S]2+ cluster | [4Fe-4S]2 metalloprotein | Thermosynechococcus vestitus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin | Thermosynechococcus vestitus | essential enzyme of the non-mevalonate (2-C-methyl-D-erythritol-4-phosphate (MEP)) pathway for isoprenoid biosynthesis | (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermosynechococcus vestitus | Q8DK70 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant GcpE protein is purified by an N-terminal His6 tag and reconstituted as a [4Fe-4S]2 metalloprotein | Thermosynechococcus vestitus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin | - |
Thermosynechococcus vestitus | (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin | - |
? | |
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin | essential enzyme of the non-mevalonate (2-C-methyl-D-erythritol-4-phosphate (MEP)) pathway for isoprenoid biosynthesis | Thermosynechococcus vestitus | (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GcpE | ambiguous | Thermosynechococcus vestitus |
General Information | Comment | Organism |
---|---|---|
metabolism | essential enzyme of the non-mevalonate (2-C-methyl-D-erythritol-4-phosphate (MEP)) pathway for isoprenoid biosynthesis | Thermosynechococcus vestitus |