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Literature summary for 1.17.4.4 extracted from

  • Chu, P.H.; Huang, T.Y.; Williams, J.; Stafford, D.W.
    Purified vitamin K epoxide reductase alone is sufficient for conversion of vitamin K epoxide to vitamin K and vitamin K to vitamin KH2 (2006), Proc. Natl. Acad. Sci. USA, 103, 19308-19313.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.17.4.4

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of VKOR in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
warfarin
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane integral protein Homo sapiens 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
 x * 21000, recombinant enzyme, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Sf9 insect cell microsomes by ultracentrifugation, followed by HPC4 affinity chromatography in presence of DHPC and reconstitution in mixed detergent micelles, method optimization Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
reconstitution of purified recombinant enzyme using 0.4% DOPC/0.4% deoxycholate/2 mM CaCl2/4 mM THP in 25 mM 3-(Nmorpholino)-propanesulfonic acid, 150 mM NaCl, and 20%glycerol at pH 7.5, method optimization Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-epoxy-2,3-dihydro-2-methyl-3-phytyl-1,4-naphthoquinone + 1,4-dithiothreitol i.e. vitamin K 2,3-epoxide Homo sapiens 2-hydroxy-2-methyl-3-phytyl-2,3-dihydronaphthoquinone + oxidized dithiothreitol i.e. vitamin K ?
2-methyl-3-phytyl-1,4-naphthoquinone + dithiothreitol i.e. vitamin K Homo sapiens vitamin K hydroquinone + oxidized dithiothreitol
-
 ?
additional information Purified vitamin K epoxide reductase alone is sufficient for conversion of vitamin K epoxide to vitamin K and vitamin K to vitamin KH2 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 21000, recombinant enzyme, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
vitamin K epoxid reductase
-
 Homo sapiens
Vitamin K reductase
-
 Homo sapiens
VKOR
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
 assay at Homo sapiens