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Literature summary for 1.17.4.2 extracted from

  • Suto, R.K.; Whalen, M.A.; Finke, R.G.
    Adenosylcobalamin-dependent ribonucleoside triphosphate reductase from Lactobacillus leichmannii. Rapid, improved purification involving dGTP-based affinity chromatography plus biophysical characterization studies demonstrating enhanced, crystallographic level purity (1999), Prep. Biochem. Biotechnol., 29, 273-309.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
5'-deoxyadenosylcobalamin
-
Lactobacillus leichmannii

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli Lactobacillus leichmannii

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Lactobacillus leichmannii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
81800
-
MALDI-TOF mass spectrometry Lactobacillus leichmannii
81850
-
predicted molecular weight based on the predicted amino acid sequence from the sequenced gene Lactobacillus leichmannii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ribonucleoside triphosphate + reduced thioredoxin + H2O Lactobacillus leichmannii
-
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r

Organism

Organism UniProt Comment Textmining
Lactobacillus leichmannii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Lactobacillus leichmannii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + reduced thioredoxin
-
Lactobacillus leichmannii dATP + oxidized thioredoxin + H2O
-
?
ribonucleoside triphosphate + reduced thioredoxin
-
Lactobacillus leichmannii 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O
-
Lactobacillus leichmannii 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
UTP + reduced thioredoxin
-
Lactobacillus leichmannii dUTP + oxidized thioredoxin + H2O
-
?

Synonyms

Synonyms Comment Organism
RTPR
-
Lactobacillus leichmannii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00367
-
CTP
-
Lactobacillus leichmannii
0.0145
-
ATP
-
Lactobacillus leichmannii
0.0158
-
UTP
-
Lactobacillus leichmannii
0.0183
-
CTP
-
Lactobacillus leichmannii
0.0233
-
CTP
-
Lactobacillus leichmannii
0.03
-
CTP
-
Lactobacillus leichmannii

Cofactor

Cofactor Comment Organism Structure
coenzyme B12 adenosylcobalamin Lactobacillus leichmannii