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Literature summary for 1.17.4.2 extracted from
- The B12-dependent ribonucleotide reductase from the archaebacterium Thermoplasma acidophila: an evolutionary solution to the ribonucleotide reductase conundrum (1997), Proc. Natl. Acad. Sci. USA, 94, 53-58.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 5'-deoxyadenosylcobalamin | - |
Thermoplasma acidophilum |  |
| 5'-deoxyadenosylcobalamin | - |
Lactobacillus leichmannii | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| chromosomal DNA containg RNR gene PCR amplified, gene cloned, completely sequenced and expressed in Escherichia coli | Thermoplasma acidophilum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 100000 | - |
size exclusion chromatography, SDS-PAGE | Thermoplasma acidophilum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ribonucleoside triphosphate + reduced thioredoxin + H2O | Thermus aquaticus | - |
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
| ribonucleoside triphosphate + reduced thioredoxin + H2O | Escherichia coli | - |
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
| ribonucleoside triphosphate + reduced thioredoxin + H2O | Homo sapiens | - |
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
| ribonucleoside triphosphate + reduced thioredoxin + H2O | Halobacterium salinarum | - |
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
| ribonucleoside triphosphate + reduced thioredoxin + H2O | Haloferax volcanii | - |
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
| ribonucleoside triphosphate + reduced thioredoxin + H2O | Thermoplasma acidophilum | - |
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
| ribonucleoside triphosphate + reduced thioredoxin + H2O | Mycobacterium tuberculosis | - |
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
| ribonucleoside triphosphate + reduced thioredoxin + H2O | Anabaena sp. | - |
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
| ribonucleoside triphosphate + reduced thioredoxin + H2O | Lactobacillus leichmannii | - |
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
| ribonucleoside triphosphate + reduced thioredoxin + H2O | Thermus aquaticus X1 | - |
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Anabaena sp. | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Halobacterium salinarum | - |
- |
- |
| Haloferax volcanii | - |
- |
- |
| Homo sapiens | - |
human | - |
| Lactobacillus leichmannii | - |
- |
- |
| Mycobacterium tuberculosis | - |
- |
- |
| Thermoplasma acidophilum | - |
DSM 1728 | - |
| Thermus aquaticus | - |
- |
- |
| Thermus aquaticus X1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermoplasma acidophilum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0018 | - |
- |
Thermoplasma acidophilum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ribonucleoside triphosphate + reduced thioredoxin | - |
Thermus aquaticus | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
| ribonucleoside triphosphate + reduced thioredoxin | - |
Escherichia coli | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
| ribonucleoside triphosphate + reduced thioredoxin | - |
Homo sapiens | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
| ribonucleoside triphosphate + reduced thioredoxin | - |
Halobacterium salinarum | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
| ribonucleoside triphosphate + reduced thioredoxin | - |
Haloferax volcanii | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
| ribonucleoside triphosphate + reduced thioredoxin | - |
Thermoplasma acidophilum | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
| ribonucleoside triphosphate + reduced thioredoxin | - |
Mycobacterium tuberculosis | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
| ribonucleoside triphosphate + reduced thioredoxin | - |
Anabaena sp. | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
| ribonucleoside triphosphate + reduced thioredoxin | - |
Lactobacillus leichmannii | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
| ribonucleoside triphosphate + reduced thioredoxin | - |
Thermus aquaticus X1 | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
| ribonucleoside triphosphate + reduced thioredoxin + H2O | - |
Thermus aquaticus | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
| ribonucleoside triphosphate + reduced thioredoxin + H2O | - |
Escherichia coli | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
| ribonucleoside triphosphate + reduced thioredoxin + H2O | - |
Homo sapiens | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
| ribonucleoside triphosphate + reduced thioredoxin + H2O | - |
Halobacterium salinarum | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
| ribonucleoside triphosphate + reduced thioredoxin + H2O | - |
Haloferax volcanii | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
| ribonucleoside triphosphate + reduced thioredoxin + H2O | - |
Thermoplasma acidophilum | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
| ribonucleoside triphosphate + reduced thioredoxin + H2O | - |
Mycobacterium tuberculosis | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
| ribonucleoside triphosphate + reduced thioredoxin + H2O | - |
Anabaena sp. | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
| ribonucleoside triphosphate + reduced thioredoxin + H2O | - |
Lactobacillus leichmannii | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
| ribonucleoside triphosphate + reduced thioredoxin + H2O | - |
Thermus aquaticus X1 | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Thermoplasma acidophilum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| coenzyme B12 | - |
Thermoplasma acidophilum | |
| coenzyme B12 | - |
Mycobacterium tuberculosis | |
| coenzyme B12 | - |
Lactobacillus leichmannii | |
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