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Literature summary for 1.17.4.1 extracted from

  • Johansson, R.; Jonna, V.R.; Kumar, R.; Nayeri, N.; Lundin, D.; Sjoeberg, B.M.; Hofer, A.; Logan, D.T.
    Structural mechanism of allosteric activity regulation in a ribonucleotide reductase with double ATP cones (2016), Structure, 24, 906-917 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ATP overall activity is stimulated by ATP and downregulated by dATP via a genetically mobile ATP cone domain mediating the formation of oligomeric complexes with varying quaternary structures Pseudomonas aeruginosa
dATP overall activity is stimulated by ATP and downregulated by dATP via a genetically mobile ATP cone domain mediating the formation of oligomeric complexes with varying quaternary structures Pseudomonas aeruginosa

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop method Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
E106A/E126A mutant enzyme completely loses the ability to be inhibited by dATP. Like the wild-type protein the mutant enzyme can bind approximately three dATP per polypeptide. The mutant enzyme loses the ability to tetramerize and only forms dimers regardless of allosteric effector Pseudomonas aeruginosa
H72A/D73A/Y830A the mutant enzyme forms inactive tetramers in the presence of any allosteric effector, the mutant enzyme partially loses its propensity to be inhibited by dATP. Like the wild-type protein the mutant enzyme can bind approximately three dATP per polypeptide Pseudomonas aeruginosa
R119D mutant enzyme completely loses the ability to be inhibited by dATP. Like the wild-type protein the mutant enzyme can bind approximately three dATP per polypeptide. The mutant enzyme loses the ability to tetramerize and only forms dimers regardless of allosteric effector Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
428000
-
tetramer, calculated from sequence Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9I4I1 NrdA
-

Subunits

Subunits Comment Organism
homotetramer 4 * 107106, calculated from sequence Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
class I RNR
-
Pseudomonas aeruginosa