Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | overall activity is stimulated by ATP and downregulated by dATP via a genetically mobile ATP cone domain mediating the formation of oligomeric complexes with varying quaternary structures | Pseudomonas aeruginosa | |
dATP | overall activity is stimulated by ATP and downregulated by dATP via a genetically mobile ATP cone domain mediating the formation of oligomeric complexes with varying quaternary structures | Pseudomonas aeruginosa |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
hanging drop method | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
E106A/E126A | mutant enzyme completely loses the ability to be inhibited by dATP. Like the wild-type protein the mutant enzyme can bind approximately three dATP per polypeptide. The mutant enzyme loses the ability to tetramerize and only forms dimers regardless of allosteric effector | Pseudomonas aeruginosa |
H72A/D73A/Y830A | the mutant enzyme forms inactive tetramers in the presence of any allosteric effector, the mutant enzyme partially loses its propensity to be inhibited by dATP. Like the wild-type protein the mutant enzyme can bind approximately three dATP per polypeptide | Pseudomonas aeruginosa |
R119D | mutant enzyme completely loses the ability to be inhibited by dATP. Like the wild-type protein the mutant enzyme can bind approximately three dATP per polypeptide. The mutant enzyme loses the ability to tetramerize and only forms dimers regardless of allosteric effector | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
428000 | - |
tetramer, calculated from sequence | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9I4I1 | NrdA | - |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 107106, calculated from sequence | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
class I RNR | - |
Pseudomonas aeruginosa |