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Literature summary for 1.17.4.1 extracted from

  • Cotruvo, J.A.; Stubbe, J.
    An active dimanganese(III)-tyrosyl radical cofactor in Escherichia coli class Ib ribonucleotide reductase (2010), Biochemistry, 49, 1297-1309.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.17.4.1

Activating Compound

Activating Compound Comment Organism Structure
dATP
-
 Escherichia coli
NrdI is an essential player in Escherichia coli class Ib RNR cluster assembly, overview. Preparation of recombinant N-terminally His6-tagged NrdI Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Escherichia coli
Mn3+ the MnIII2-tyrosyl radical cofactor, not the diferric-tyrosyl radical one, is the active metallocofactor in vivo Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Subunits

Subunits Comment Organism
tetramer class Ib RNR is composed of two homodimeric subunits: alpha2 or NrdE, where nucleotide reduction occurs, and beta2 or NrdF, which contains an unidentified metallocofactor that initiates nucleotide reduction Escherichia coli

Synonyms

Synonyms Comment Organism
class Ib ribonucleotide reductase
-
 Escherichia coli
class Ib RNR
-
 Escherichia coli
NrdE
-
 Escherichia coli
NrdF
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
dimanganese(III)-tyrosyl radical cofactor the dimanganese(III)-tyrosyl radical cofactor, not the diferric-tyrosyl radical one, is the active metallocofactor in vivo Escherichia coli
additional information analysis of composition and conformation, and of the reaction mechanism of the metallo-cofactor, detailed overview Escherichia coli

Expression

Organism Comment Expression
Escherichia coli class Ib RNR is expressed under iron-limited and oxidative stress conditions up

General Information

General Information Comment Organism
physiological function Escherichia coli class Ib RNR converts nucleoside 5'-diphosphates to deoxynucleoside 5'-diphosphates and is expressed under iron-limited and oxidative stress conditions Escherichia coli