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Literature summary for 1.17.4.1 extracted from

  • Smith, P.; Zhou, B.; Ho, N.; Yuan, Y.C.; Su, L.; Tsai, S.C.; Yen, Y.
    2.6 a X-ray crystal structure of human p53R2, a p53-inducible ribonucleotide reductase (2009), Biochemistry, 48, 11134-11141.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
P53 activates, required Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His6-tagged hp53R2, sitting drop vapor diffusion method, at 25°C, 0.002 ml of 4.5 mg/ml protein in 20 mM Tris, pH 7.5, are mixed with 150 mM NaCl and 0.002 ml of precipitant solution containing 0.1 M sodium citrate, pH 6.45, 1.3 M Li2SO4, and 0.5 M (NH4)2SO4, reservoir volume is 0.250 ml, 7-14 days, addition of ferrous ammonium sulfate of 5 mM 1 h prior to harvesting, X-ray diffraction structure determination and analysis at 2.6 A resolution Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
deferoxamine mesylate an iron chelator Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ monomers A and B exhibit mono- and binuclear iron occupancy, the active site iron coordination environment, involving E131, H134, D100, E194, E228, and H231, is different between monomers A and B, binding structure, overview. Mobility and accessibility of the radical iron center, and radical transfer pathway, overview Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens human p53R2 is a 351-residue p53-inducible ribonucleotide reductase small subunit, hp53R2 supplies dNTPs for DNA repair to cells in G0-G1 in a p53-dependent fashion, rather than exhibiting cyclic dNTP synthesis. Hp53R2 structure-function relationship determination and analysis, overview ?
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Organism

Organism UniProt Comment Textmining
Homo sapiens
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Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information human p53R2 is a 351-residue p53-inducible ribonucleotide reductase small subunit, hp53R2 supplies dNTPs for DNA repair to cells in G0-G1 in a p53-dependent fashion, rather than exhibiting cyclic dNTP synthesis. Hp53R2 structure-function relationship determination and analysis, overview Homo sapiens ?
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?

Subunits

Subunits Comment Organism
More one monomer can swivel between two conformations and impose significant influences on helix D and helix B of the opposite monomer. This change ultimately affects the orientation of D100 and, thus, the integrity of the binuclear iron environment. Structural basis of B helix disorder and the N-terminal swivel region, overview Homo sapiens

Synonyms

Synonyms Comment Organism
p53-inducible ribonucleotide reductase
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Homo sapiens
RNR
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Homo sapiens

General Information

General Information Comment Organism
physiological function hp53R2 possibly plays a regulatory role in iron assimilation Homo sapiens