Activating Compound | Comment | Organism | Structure |
---|---|---|---|
P53 | activates, required | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His6-tagged hp53R2, sitting drop vapor diffusion method, at 25°C, 0.002 ml of 4.5 mg/ml protein in 20 mM Tris, pH 7.5, are mixed with 150 mM NaCl and 0.002 ml of precipitant solution containing 0.1 M sodium citrate, pH 6.45, 1.3 M Li2SO4, and 0.5 M (NH4)2SO4, reservoir volume is 0.250 ml, 7-14 days, addition of ferrous ammonium sulfate of 5 mM 1 h prior to harvesting, X-ray diffraction structure determination and analysis at 2.6 A resolution | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
deferoxamine mesylate | an iron chelator | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | monomers A and B exhibit mono- and binuclear iron occupancy, the active site iron coordination environment, involving E131, H134, D100, E194, E228, and H231, is different between monomers A and B, binding structure, overview. Mobility and accessibility of the radical iron center, and radical transfer pathway, overview | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | human p53R2 is a 351-residue p53-inducible ribonucleotide reductase small subunit, hp53R2 supplies dNTPs for DNA repair to cells in G0-G1 in a p53-dependent fashion, rather than exhibiting cyclic dNTP synthesis. Hp53R2 structure-function relationship determination and analysis, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | human p53R2 is a 351-residue p53-inducible ribonucleotide reductase small subunit, hp53R2 supplies dNTPs for DNA repair to cells in G0-G1 in a p53-dependent fashion, rather than exhibiting cyclic dNTP synthesis. Hp53R2 structure-function relationship determination and analysis, overview | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | one monomer can swivel between two conformations and impose significant influences on helix D and helix B of the opposite monomer. This change ultimately affects the orientation of D100 and, thus, the integrity of the binuclear iron environment. Structural basis of B helix disorder and the N-terminal swivel region, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
p53-inducible ribonucleotide reductase | - |
Homo sapiens |
RNR | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | hp53R2 possibly plays a regulatory role in iron assimilation | Homo sapiens |