Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | ATP is an allosteric effector | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
Y730F | site-directed mutagenesis, the mutant excludes a direct superexchange mechanism between C439 and Y731 in radical transport, overview | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
peptide Y-R2C19 | a 20-mer peptide, which is identical to the C-terminal peptide tail of the R2 subunit and is a known competitive inhibitor of binding of the native R2 protein to R1 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP + thioredoxin | Escherichia coli | - |
2'-deoxyCDP + thioredoxin disulfide + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2'-deoxyribonucleoside 5'-diphosphate + thioredoxin disulfide + H2O = ribonucleoside 5'-diphosphate + thioredoxin | reaction mechanism via formation of a tyrosyl radical at Tyr356 od subunit R1, model for radical transport in which there is a unidirectional transport of the electron and proton transport among residues of R1 | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP + thioredoxin | - |
Escherichia coli | 2'-deoxyCDP + thioredoxin disulfide + H2O | - |
? | |
CDP + thioredoxin | coupled assay method with NADPH, transient spectrometric measurement of Y356 radical intermediate, Y356 radical initiation is prompted by excitation of a proximal anthraquinone or benzophenone chromophore on a 20-mer peptide Y-R2C19, bound to subunit alpha2, both the Anq and BPA-containing peptides are competent in deoxycytidine diphosphate formation and turnover occurs via Y731 to Y730 to C439 pathway-dependent radical transport in R1, overview. Peptide Y-R2C19 is identical to the C-terminal peptide tail of the R2 subunit and is a known competitive inhibitor of binding of the native R2 protein to R1 | Escherichia coli | 2'-deoxyCDP + thioredoxin disulfide + H2O | - |
? | |
additional information | mechanism of radical transport in the R1 subunit of the class I enzyme, overview | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 2 * subunit R1 + 2 * subunit R2 | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
More | class I ribonucleotide reductase | Escherichia coli |
RNR | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thioredoxin | - |
Escherichia coli |