Cloned (Comment) | Organism |
---|---|
expression of Y122F, Y356F and Y122F/Y356F mutant enzymes in Escherichia coli | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
Y122F | mutant enzyme cannot generate a Y122 tyrosyl radical necessary for catalysis, 0.5% of wild-type activity | Escherichia coli |
Y122F/Y356F | 0.5% of wild-type activity | Escherichia coli |
Y356F | similar properties as wild-type | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | proposed in vitro mechanism for the assembly of the diferric tyrosyl radical cofactor of subunit R2 | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P69924 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ribonucleoside diphosphate + reduced thioredoxin | - |
Escherichia coli | 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O | - |
ir |
Subunits | Comment | Organism |
---|---|---|
More | proposed in vitro mechanism for the assembly of the diferric tyrosyl radical cofactor of subunit R2 | Escherichia coli |