Any feedback?
Literature summary for 1.17.4.1 extracted from
- Rat liver ribonucleotide reductase: separation, purification, and properties of two nonidentical subunits (1982), Can. J. Biochem., 60, 463-470.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.09 | - |
CDP | subunit L1 | Rattus norvegicus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | 120000 Da L2 subunit of regenerating liver contains iron | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
4 * 45000 + 1 * 45000 + 1 * 75000, regenerating liver | Rattus norvegicus |
| 45000 | - |
x * 45000 + 1 * 75000 + 1 * 45000, holoenzyme may have an alpha4,beta,beta structure, SDS-PAGE | Rattus norvegicus |
| 75000 | - |
4 * 45000 + 1 * 45000 + 1 * 75000, regenerating liver | Rattus norvegicus |
| 75000 | - |
x * 45000 + 1 * 75000 + 1 * 45000, holoenzyme may have an alpha4,beta,beta structure, SDS-PAGE | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| regenerating liver, dATP-Sepharose affinity chromatography | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | regenerating liver | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0131 | - |
purified subunit L2 assayed in the presenc of L1 | Rattus norvegicus |
| 0.015 | - |
purified subunit L1 assayed in the presenc of L2, specific for CDP | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, 0.25 M sucrose, 100 mM Tris-HCl, pH 7.6, 10 mM MgCl2, 2 mM dithiothreitol, 1 month, no loss of activity | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ribonucleoside diphosphate + reduced thioredoxin | - |
Rattus norvegicus | 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000 + 1 * 75000 + 1 * 45000, holoenzyme may have an alpha4,beta,beta' structure, SDS-PAGE | Rattus norvegicus |
| hexamer | 4 * 45000 + 1 * 45000 + 1 * 75000, regenerating liver | Rattus norvegicus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
