Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | stimulation of UDP reduction | Escherichia coli | |
ATP | stimulation of CDP reduction | Escherichia coli | |
dTTP | stimulation of UDP reduction | Escherichia coli | |
dTTP | stimulation of CDP reduction | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C225A | 4-6% of wild-type activity | Escherichia coli |
C225S | major product formed by interaction with CDP is cytosine | Escherichia coli |
C439A | 4-6% of wild-type activity | Escherichia coli |
C462S | in the presence of dithiothreitol the major product formed by interaction with CDP is cytosine | Escherichia coli |
C754A | active with dithiothreitol as reductant, 3% of wild type activity with thioredoxin | Escherichia coli |
C759A | active with dithiothreitol as reductant, 3% of wild type activity with thioredoxin | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CDP | competitive inhibition of UDP reduction | Escherichia coli | |
dATP | inhibition of CDP reduction; inhibition of UDP reduction | Escherichia coli | |
additional information | mechanism-based inhibitors | Escherichia coli | |
UDP | competitive inhibition of CDP reduction | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | B2 subunit contains 2 dinuclear Fe3+ centers | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2'-deoxyribonucleoside 5'-diphosphate + thioredoxin disulfide + H2O = ribonucleoside 5'-diphosphate + thioredoxin | proposed mechanism | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ribonucleoside diphosphate + reduced thioredoxin | - |
Escherichia coli | 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O | - |
ir |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.33 | - |
CDP | activity of subunit B1 assayed in the presence of an excess of subunit B2 | Escherichia coli | |
12.9 | - |
CDP | activity of subunit B2 assayed in the presence of an excess of subunit B1 | Escherichia coli |