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Literature summary for 1.17.4.1 extracted from
- Ribonucleotide reductases: amazing and confusing (1990), J. Biol. Chem., 265, 5329-5332.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ATP | stimulation of UDP reduction | Escherichia coli |  |
| ATP | stimulation of CDP reduction | Escherichia coli | |
| dTTP | stimulation of UDP reduction | Escherichia coli | |
| dTTP | stimulation of CDP reduction | Escherichia coli | |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C225A | 4-6% of wild-type activity | Escherichia coli |
| C225S | major product formed by interaction with CDP is cytosine | Escherichia coli |
| C439A | 4-6% of wild-type activity | Escherichia coli |
| C462S | in the presence of dithiothreitol the major product formed by interaction with CDP is cytosine | Escherichia coli |
| C754A | active with dithiothreitol as reductant, 3% of wild type activity with thioredoxin | Escherichia coli |
| C759A | active with dithiothreitol as reductant, 3% of wild type activity with thioredoxin | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CDP | competitive inhibition of UDP reduction | Escherichia coli | |
| dATP | inhibition of CDP reduction; inhibition of UDP reduction | Escherichia coli | |
| additional information | mechanism-based inhibitors | Escherichia coli | |
| UDP | competitive inhibition of CDP reduction | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | B2 subunit contains 2 dinuclear Fe3+ centers | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 2'-deoxyribonucleoside 5'-diphosphate + thioredoxin disulfide + H2O = ribonucleoside 5'-diphosphate + thioredoxin | proposed mechanism | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ribonucleoside diphosphate + reduced thioredoxin | - |
Escherichia coli | 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O | - |
ir | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.33 | - |
CDP | activity of subunit B1 assayed in the presence of an excess of subunit B2 | Escherichia coli | |
| 12.9 | - |
CDP | activity of subunit B2 assayed in the presence of an excess of subunit B1 | Escherichia coli | |
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