Cloned (Comment) | Organism |
---|---|
overexpression of B2 subunit | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
chimeric R2 genes in which C-terminal sequences in Escherichia coli subunit R2 are replaced by C-terminal sequences from the mouse subunit R2 | Escherichia coli |
recombinant B2 subunit, Sephadex G-25, DEAE Bio gel, Sephadex QAE-50 | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.21 | - |
recombinant subunit B2 | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ribonucleoside diphosphate + reduced thioredoxin | - |
Escherichia coli | 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O | - |
ir |