Literature summary for 1.17.4.1 extracted from

Hards, R.G.; Wright, J.A.
Ribonucleotide reductase activity in intact mammalian cells: stimulation of enzyme activity by MgCl2, dithiothreitol, and several nucleotides (1984), Arch. Biochem. Biophys., 231, 9-16.

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Activating Compound

Activating Compound	Comment	Organism	Structure
ATP	stimulation of CDP reduction	Cricetulus griseus
ATP	stimulation of ADP reduction	Cricetulus griseus
dCTP	stimulation of CDP and ADP reduction	Cricetulus griseus
dGTP	stimulation of CDP reduction	Cricetulus griseus
dithiothreitol	required for optimal activity	Cricetulus griseus
GTP	stimulation of CDP and ADP reduction	Cricetulus griseus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.021	-	CDP	in the presence of 1.5 mM ATP	Cricetulus griseus
0.033	-	CDP	in the presence of 3.6 mM dCTP	Cricetulus griseus
0.095	-	ADP	optimum dGTP concentration	Cricetulus griseus
0.2	-	ADP	in the presence of 0.2 mM GTP	Cricetulus griseus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	stimulates CDP but not ADP reduction	Cricetulus griseus

Organism

Organism	UniProt	Comment	Textmining
Cricetulus griseus	-	Chinese hamster overy cells	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
CHO cell	-	Cricetulus griseus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ADP + reduced dithiothreitol	-	Cricetulus griseus	2'-dADP + oxidized dithiothreitol + H2O	-	r
CDP + reduced dithiothreitol	-	Cricetulus griseus	2'-dCDP + oxidized dithiothreitol + H2O	-	r
ribonucleoside diphosphate + reduced thioredoxin	-	Cricetulus griseus	2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O	-	ir

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Release 2023.1 (January 2023)