Literature summary for 1.17.4.1 extracted from

Averett, D.R.; Lubbers, C.; Elion, G.B.; Spector, T.
Ribonucleotide reductase induced by herpes simplex type 1 virus. Characterization of a distinct enzyme (1983), J. Biol. Chem., 258, 9831-9838.

Search for more literature for 1.17.4.1

Inhibitors

Inhibitors	Comment	Organism	Structure
3,5-diamino-1H-1,2,4-triazole	2.3 mM, 50% inhibition of CDP reduction, 2.6 mM, 50% inhibition of ADP reduction	Mesocricetus auratus
ADP	-	Mesocricetus auratus
ATP	CDP reduction is inhibited by free ATP	Mesocricetus auratus
CDP	-	Mesocricetus auratus
dADP	product inhibition	Mesocricetus auratus
dATP	2.1 mM, 50% inhibition; inhibition of CDP reduction; weak inhibition of ADP reduction	Mesocricetus auratus
dCDP	product inhibition	Mesocricetus auratus
dCTP	1.2 mM, 50% inhibition of CDP reduction, 0.89 mM, 50% inhibition of ADP reduction	Mesocricetus auratus
dGDP	product inhibition	Mesocricetus auratus
dGTP	1.2 mM, 50% inhibition of CDP reduction, 0.93 mM, 50% inhibition of ADP reduction	Mesocricetus auratus
dTTP	inhibition of CDP reduction	Mesocricetus auratus
dUDP	product inhibition	Mesocricetus auratus
EDTA	-	Mesocricetus auratus
GDP	-	Mesocricetus auratus
Hydroxyurea	-	Mesocricetus auratus
Mg2+	uncomplexed Mg2+	Mesocricetus auratus
additional information	each ribonucleoside diphosphate substrate is competitively inhibited by reduction of each other substrate	Mesocricetus auratus
UDP	-	Mesocricetus auratus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00065	-	CDP	-	Mesocricetus auratus
0.0012	-	GDP	-	Mesocricetus auratus
0.012	-	ADP	-	Mesocricetus auratus
0.08	-	UDP	-	Mesocricetus auratus

Organism

Organism	UniProt	Comment	Textmining
Mesocricetus auratus	-	baby hamster kidney cells, enzyme induced by Herpes simplex, virus type 1, HSV-1	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
BHK-21 cell	-	Mesocricetus auratus	-
kidney	baby hamster kidney cells	Mesocricetus auratus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ADP + thioredoxin	-	Mesocricetus auratus	2'-dADP + thioredoxin disulfide + H2O	-	r
CDP + thioredoxin	-	Mesocricetus auratus	2'-dCDP + thioredoxin disulfide + H2O	-	r
GDP + thioredoxin	-	Mesocricetus auratus	2'-dGDP + thioredoxin disulfide + H2O	-	r
ribonucleoside diphosphate + reduced thioredoxin	-	Mesocricetus auratus	2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O	-	ir
UDP + reduced thioredoxin	-	Mesocricetus auratus	2'-deoxy-UDP + oxidized thioredoxin + H2O	-	?

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.1	-	-	Mesocricetus auratus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00058	-	CDP	vs. GDP	Mesocricetus auratus
0.0006	-	GDP	vs. ADP	Mesocricetus auratus
0.0007	-	CDP	vs. ADP	Mesocricetus auratus
0.0012	-	GDP	vs. CDP	Mesocricetus auratus
0.0018	-	GDP	vs. UDP	Mesocricetus auratus
0.0019	-	CDP	vs. UDP	Mesocricetus auratus
0.0055	-	ADP	vs. GDP	Mesocricetus auratus
0.0058	-	UDP	vs. GDP	Mesocricetus auratus
0.014	-	ADP	vs. CDP	Mesocricetus auratus
0.036	-	ADP	vs. UDP	Mesocricetus auratus
0.042	-	UDP	vs. CDP	Mesocricetus auratus
0.043	-	UDP	vs. ADP	Mesocricetus auratus

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Release 2023.1 (January 2023)