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Literature summary for 1.17.4.1 extracted from

  • Kucera, R.; Paulus, H.
    Studies on ribonucleoside-diphosphate reductase in permeable animal cells. II. Catalytic and regulatory properties of the enzyme in mouse L cells (1982), Arch. Biochem. Biophys., 214, 114-123.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
adenyl-5'-yl-imidodiphosphate maximal activation of CDP reduction at 4 mM Mus musculus
ATP reduction of CDP is dependent on ATP or adenyl-5'-yl iminodiphosphate Mus musculus
dihydrolipoic acid slight stimulation Mus musculus
dithiothreitol slight activation Mus musculus
dTTP stimulation of GDP reduction Mus musculus
EDTA reversible stimulation of GDP reduction, irreversible inhibition of CDP reduction Mus musculus
additional information enzyme of cells first treated with 2,6-dichlorophenolindophenol has a complete dependence on NADPH which can also be met by dithiothreitol or dihydrolipoic acid Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
ATP 4 mM, 50% inhibition of GDP reduction in the presence of dTTP Mus musculus
bathophenanthroline sulfonate 5 mM, almost complete inhibition of CDP and GDP reduction Mus musculus
dATP 0.0033 mM, 50% inhibition of CDP reduction, 0.0036 mM, 50% inhibition of GDP reduction; inhibition of CDP reduction Mus musculus
dCTP 1 mM, 50% inhibition of CDP reduction Mus musculus
dGTP 0.08 mM, 50% inhibition of CDP reduction, 0.19 mM, 50% inhibition of GDP reduction; inhibition of CDP reduction Mus musculus
dTTP 0.2 mM, 50% inhibition of CDP reduction Mus musculus
EDTA reversible stimulation of GDP reduction, irreversible inhibition of CDP reduction Mus musculus
Hydroxyurea 1 mM, 98 and 81% inhibition of CDP and GDP reduction respectively Mus musculus
Mg2+ 1-5 mM, 10-20% inhibition of GDP reduction; inhibition of CDP reduction Mus musculus
sodium arsenite 0.025 mM, almost complete inhibition of CDP reduction, 86% inhibition of GDP reduction Mus musculus
Thenoyltrifluoroacetone 5 mM, almost complete inhibition of CDP and GDP reduction Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
CDP
-
Mus musculus
0.24
-
GDP
-
Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Novikoff ascites tumor cells, partial Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + reduced thioredoxin
-
Mus musculus 2'-deoxy-ADP + oxidized thioredoxin + H2O
-
ir
CDP + reduced thioredoxin
-
Mus musculus 2'-deoxy-CDP + oxidized thioredoxin + H2O
-
ir
GDP + reduced thioredoxin
-
Mus musculus 2'-deoxy-GDP + oxidized thioredoxin + H2O
-
ir
ribonucleoside diphosphate + reduced thioredoxin
-
Mus musculus 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
ir
UDP + reduced thioredoxin
-
Mus musculus 2'-deoxy-UDP + oxidized thioredoxin + H2O
-
ir

Cofactor

Cofactor Comment Organism Structure
NADPH slight stimulation Mus musculus