Protein Variants | Comment | Organism |
---|---|---|
E1261X | mutation of Glu1261 leads to a complete loss of activity | Bos taurus |
E802Q | site-directed mutagenesis, altered kinetics of the mutant enzyme compared to the wild-type enzyme | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0552 | - |
xanthine | pH not specified in the publication, temperature not specified in the publication, mutant E802Q | Bos taurus | |
0.0644 | - |
xanthine | pH not specified in the publication, temperature not specified in the publication, wild-type enzyme | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | Fe2S2 cluster | Bos taurus | |
Molybdenum | molybdopterin cofactor | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xanthine + H2O + O2 | Bos taurus | - |
urate + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
xanthine + H2O + O2 = urate + H2O2 | favored mechanism for the reaction with xanthine, overview | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
milk | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-6-methylpurine + H2O + O2 | low activity | Bos taurus | ? + H2O2 | - |
? | |
additional information | quantum mechanical/molecular mechanical study of the reductive half-reaction of wild-type xanthine oxidase, overview | Bos taurus | ? | - |
? | |
xanthine + H2O + O2 | - |
Bos taurus | urate + H2O2 | - |
? | |
xanthine + H2O + O2 | effects of variations in the cofactor, the substrate, and the active site residue Glu802 on the reaction mechanism, overview | Bos taurus | urate + H2O2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.16 | - |
xanthine | pH not specified in the publication, temperature not specified in the publication, mutant E802Q | Bos taurus | |
108 | - |
xanthine | pH not specified in the publication, temperature not specified in the publication, wild-type enzyme | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
molybdenum cofactor | [Mo(S2C2H2)(dO) (ORunfixed)(sSH)]2- moiety | Bos taurus |