Cloned (Comment) | Organism |
---|---|
gene XO, DNA and amino acid sequence determination and analysis of wild-type and mutant enzymes, genotyping, expression in COS-7 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C1318Y | naturally occuring single nucleotide polymorphism, the mutant variant shows reduced activity compared to the wild-type enzyme | Homo sapiens |
D1109T | naturally occuring nonsynonymous single nucleotide polymorphism | Homo sapiens |
G172R | naturally occuring nonsynonymous single nucleotide polymorphism | Homo sapiens |
H1221R | naturally occuring nonsynonymous single nucleotide polymorphism, the mutant variant shows about twofold increased activity compared to the wild-type enzyme | Homo sapiens |
I703V | naturally occuring single nucleotide polymorphism, the mutant variant shows abput twofold increased activity compared to the wild-type enzyme | Homo sapiens |
additional information | enzyme mutantions can lead to xanthinuria | Homo sapiens |
N909K | naturally occuring single nucleotide polymorphism, the mutant variant shows reduced activity compared to the wild-type enzyme | Homo sapiens |
P1150R | naturally occuring single nucleotide polymorphism, the mutant variant shows reduced activity compared to the wild-type enzyme | Homo sapiens |
P555S | naturally occuring single nucleotide polymorphism, the mutant variant shows reduced activity compared to the wild-type enzyme | Homo sapiens |
R149C | naturally occuring single nucleotide polymorphism, inactive mutant | Homo sapiens |
R607Q | naturally occuring single nucleotide polymorphism, the mutant variant shows reduced activity compared to the wild-type enzyme | Homo sapiens |
T109K | naturally occuring single nucleotide polymorphism, inactive mutant | Homo sapiens |
T623I | naturally occuring single nucleotide polymorphism, the mutant variant shows reduced activity compared to the wild-type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | detailed kinetics of wild-type in comparison to mutant enzymes, overview | Homo sapiens | |
0.0017 | - |
xanthine | pH 8.1, 37°C, average value, wild-type enzyme | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the enzyme catalyzes the oxidation of endogenous and exogenous purines and pyrimidines | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
gene XO | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activities of wild-type in comparison to mutant enzymes, overview | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme catalyzes the oxidation of endogenous and exogenous purines and pyrimidines | Homo sapiens | ? | - |
? | |
additional information | single nucleotide polymorphisms alter the substrate specificity of the enzyme, overview | Homo sapiens | ? | - |
? | |
xanthine + H2O + O2 | - |
Homo sapiens | urate + H2O2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.1 | - |
assay at | Homo sapiens |