Crystallization (Comment) | Organism |
---|---|
xanthine oxidoreductase mutant W335A/F336L, showing two similar, but not identical subunits. The cluster involved in conformation-switching is completely disrupted in one subunit, but remains partly associated in the other. Xanthine oxidase and oxidoreductase forms of the mutant are in equilibrium that greatly favors the oxidase form, but upon incubation with dithiothreitol equilibrium is partly shifted towards the oxidoreductase form | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
W335A/F336L | xanthine oxidoreductase mutant displaying xanthine oxidase activity | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.129 | - |
O2 | xanthine oxidoreductase mutant W335A/F336L treated with dithiothreitol, pH 7.8, 25°C | Rattus norvegicus | |
0.129 | - |
O2 | xanthine oxidoreductase mutant W335A/F336L, pH 7.8, 25°C | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P22985 | conversion of xanthine oxidoreductase to oxidase | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
xanthine + H2O + O2 | - |
Rattus norvegicus | urate + H2O2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
xanthine | xanthine oxidoreductase mutant W335A/F336L, pH 7.8, 25°C | Rattus norvegicus | |
9.73 | - |
xanthine | xanthine oxidoreductase mutant W335A/F336L treated with dithiothreitol, pH 7.8, 25°C | Rattus norvegicus | |
12.47 | - |
xanthine | xanthine oxidoreductase mutant W335A/F336L, pH 7.8, 25°C | Rattus norvegicus |