Literature summary for 1.17.1.9 extracted from

Wang, R.; Zeng, Z.; Guo, H.; Tan, H.; Liu, A.; Zhao, Y.; Chen, L.
Over-expression of the Arabidopsis formate dehydrogenase in chloroplasts enhances formaldehyde uptake and metabolism in transgenic tobacco leaves (2018), Planta, 247, 339-354 .

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Niotiana tabacum leaf chloroplasts	Arabidopsis thaliana
overexpression in the chloroplasts of transgenic tobacco using a light inducible promote. Overexpression of the Arabidopsis formate dehydrogenase in chloroplasts enhances formaldehyde uptake and metabolism in transgenic tobacco leaves. Overexpression in chloroplasts fails to introduce the carbon flux of HCHO metabolism into the Calvin cycle in transgenic tobacco leaves. However, the overexpression significantly enhances the HCHO metabolism in transgenic leaves. Consequently, the productions of Asn, Gln, oxalate, and HCOOH are notably greater in transgenic leaves than in non-transformed leaves after treatment with HcHO. The increased stomatal conductance and aperture in transgenic leaves might be ascribed to the increased yield of oxalate in the guard cells with overexpressed enzyme in chloroplasts. Accordingly, the transgenic plants exhibit a stronger capacity to absorb gaseous HCHO. The higher proline content in transgenic leaves compared with non-transformed leaves under HCHO stress might be attributable to the excess formate accumulation and Gln production. Consequently, the HCHO-induced oxidative stress is reduced in transgenic leaves	Arabidopsis thaliana

Cloned (Comment)

Organism

expressed in Niotiana tabacum leaf chloroplasts

Arabidopsis thaliana

overexpression in the chloroplasts of transgenic tobacco using a light inducible promote. Overexpression of the Arabidopsis formate dehydrogenase in chloroplasts enhances formaldehyde uptake and metabolism in transgenic tobacco leaves. Overexpression in chloroplasts fails to introduce the carbon flux of HCHO metabolism into the Calvin cycle in transgenic tobacco leaves. However, the overexpression significantly enhances the HCHO metabolism in transgenic leaves. Consequently, the productions of Asn, Gln, oxalate, and HCOOH are notably greater in transgenic leaves than in non-transformed leaves after treatment with HcHO. The increased stomatal conductance and aperture in transgenic leaves might be ascribed to the increased yield of oxalate in the guard cells with overexpressed enzyme in chloroplasts. Accordingly, the transgenic plants exhibit a stronger capacity to absorb gaseous HCHO. The higher proline content in transgenic leaves compared with non-transformed leaves under HCHO stress might be attributable to the excess formate accumulation and Gln production. Consequently, the HCHO-induced oxidative stress is reduced in transgenic leaves

Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast	-	Arabidopsis thaliana	9507	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
formate + NAD+	Arabidopsis thaliana	-	CO2 + NADH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	-	-
Arabidopsis thaliana	Q9S7E4	-	-
Arabidopsis thaliana cv. Xanthi	Q9S7E4	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Arabidopsis thaliana	-
seedling	-	Arabidopsis thaliana	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
formate + NAD+	-	Arabidopsis thaliana	CO2 + NADH + H+	-	?
formate + NAD+	-	Arabidopsis thaliana cv. Xanthi	CO2 + NADH + H+	-	?

Subunits

Subunits	Comment	Organism
?	x * 42000, SDS-PAGE	Arabidopsis thaliana

Synonyms

Synonyms	Comment	Organism
FDH	-	Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Arabidopsis thaliana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Arabidopsis thaliana

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Arabidopsis thaliana

General Information

General Information	Comment	Organism
metabolism	formate dehydrogenase is a key enzyme in formaldehyde metabolism by oxidation of HCOOH to CO2, which enters the Calvin cycle to be assimilated into glucose	Arabidopsis thaliana
physiological function	the enzyme increases formaldehyde uptake and resistance in transgenic tobacco leaves	Arabidopsis thaliana