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Literature summary for 1.17.1.9 extracted from

  • Carter, J.L.; Bekhouche, M.; Noiriel, A.; Blum, L.J.; Doumeche, B.
    Directed evolution of a formate dehydrogenase for increased tolerance to ionic liquids reveals a new site for increasing the stability (2014), ChemBioChem, 15, 2710-2718.
    View publication on PubMed
Search for more literature for 1.17.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells [Candida] boidinii

Protein Variants

Protein Variants Comment Organism
N187S the mutation is responsible for improved activity through an enhancement of the kcat value by a factor of 5.8 [Candida] boidinii
N187S/T321S the mutation is responsible for improved activity through an enhancement of the kcat value by a factor of 6.7 [Candida] boidinii
T321S the mutation is responsible for improved activity through an enhancement of the kcat value by a factor of 1.8 [Candida] boidinii

General Stability

General Stability Organism
the wild type enzyme unfolds in the presence of 50% (v/v) 1,3-dimethylimidazolium dimethyl phosphate [Candida] boidinii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.037
-
 formate mutant enzyme N187S/T321S, at pH 7.2 and 30°C [Candida] boidinii
0.075
-
 formate wild type enzyme, at pH 7.2 and 30°C [Candida] boidinii
0.08
-
 formate mutant enzyme N187S, at pH 7.2 and 30°C [Candida] boidinii
0.084
-
 formate mutant enzyme T321S, at pH 7.2 and 30°C [Candida] boidinii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formate + NAD+ [Candida] boidinii
-
 CO2 + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
[Candida] boidinii O13437
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + NAD+
-
 [Candida] boidinii CO2 + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
FDH
-
 [Candida] boidinii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 after 2 h at 40°C and 60°C, the wild type enzyme retains 44% and 10% of its initial activity, respectively [Candida] boidinii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.21
-
 formate wild type enzyme, at pH 7.2 and 30°C [Candida] boidinii
0.44
-
 formate mutant enzyme T321S, at pH 7.2 and 30°C [Candida] boidinii
0.71
-
 formate mutant enzyme N187S/T321S, at pH 7.2 and 30°C [Candida] boidinii
1.25
-
 formate mutant enzyme N187S, at pH 7.2 and 30°C [Candida] boidinii

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 [Candida] boidinii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.8
-
 formate wild type enzyme, at pH 7.2 and 30°C [Candida] boidinii
5.1
-
 formate mutant enzyme T321S, at pH 7.2 and 30°C [Candida] boidinii
15.5
-
 formate mutant enzyme N187S, at pH 7.2 and 30°C [Candida] boidinii
19
-
 formate mutant enzyme N187S/T321S, at pH 7.2 and 30°C [Candida] boidinii